Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors
Protein‐protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challeng...
| Principais autores: | , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Wiley
2019
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| _version_ | 1826282519501209600 |
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| author | Bery, N Rabbitts, TH |
| author_facet | Bery, N Rabbitts, TH |
| author_sort | Bery, N |
| collection | OXFORD |
| description | Protein‐protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challenging to convert to therapeutics. In the last decade, much progress has been made in the inhibition of PPIs involved in diseases, but many remain difficult such as RAS‐effector interactions in cancers. We describe here a protocol for using Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to detect and characterize RAS PPI inhibition by macromolecules and small molecules. This method could be extended to any other small GTPases or any other PPIs of interest. |
| first_indexed | 2024-03-07T00:45:05Z |
| format | Journal article |
| id | oxford-uuid:845a6c07-932f-4bc2-a63f-62e68c5980c2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:45:05Z |
| publishDate | 2019 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:845a6c07-932f-4bc2-a63f-62e68c5980c22022-03-26T21:50:41ZBioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitorsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:845a6c07-932f-4bc2-a63f-62e68c5980c2EnglishSymplectic Elements at OxfordWiley2019Bery, NRabbitts, THProtein‐protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance of PPIs in biology, this target class has been very challenging to convert to therapeutics. In the last decade, much progress has been made in the inhibition of PPIs involved in diseases, but many remain difficult such as RAS‐effector interactions in cancers. We describe here a protocol for using Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to detect and characterize RAS PPI inhibition by macromolecules and small molecules. This method could be extended to any other small GTPases or any other PPIs of interest. |
| spellingShingle | Bery, N Rabbitts, TH Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title | Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title_full | Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title_fullStr | Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title_full_unstemmed | Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title_short | Bioluminescence Resonance Energy Transfer 2 (BRET2)‐based RAS biosensors to characterize RAS inhibitors |
| title_sort | bioluminescence resonance energy transfer 2 bret2 based ras biosensors to characterize ras inhibitors |
| work_keys_str_mv | AT beryn bioluminescenceresonanceenergytransfer2bret2basedrasbiosensorstocharacterizerasinhibitors AT rabbittsth bioluminescenceresonanceenergytransfer2bret2basedrasbiosensorstocharacterizerasinhibitors |