Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells.
A recent study reported that quantitation of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in the graft and monitoring of these T cells might identify hematopoietic stem cell transplantation-recipients at the risk for progressive CMV infection. A 6-year-old girl underwent bone marrow transplanta...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2006
|
| _version_ | 1797079473520115712 |
|---|---|
| author | Komatsu, H Kogawa, K Nonoyama, S Inui, A Sogo, T Fujisawa, T Klenerman, P |
| author_facet | Komatsu, H Kogawa, K Nonoyama, S Inui, A Sogo, T Fujisawa, T Klenerman, P |
| author_sort | Komatsu, H |
| collection | OXFORD |
| description | A recent study reported that quantitation of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in the graft and monitoring of these T cells might identify hematopoietic stem cell transplantation-recipients at the risk for progressive CMV infection. A 6-year-old girl underwent bone marrow transplantation from an HLA-identical sibling with a very high frequency of CMV specific tetramer-positive CD8+ T-cells. CMV-specific T-cell immunity was prospectively evaluated using a peptide (HLA-A2, NLVPMVATV). Tetramer assay showed that the frequency of CMV-specific CD8+ T cells of the donor in the peripheral blood was 5.3%, higher than average amongst young children. The frequency of CMV-specific CD8+ T cells of the donor in the graft was 3.7% of CD8+ T-cells. Before transplantation, the frequency of CMV specific CD8+ T cells of the recipient was 0.1% in the peripheral blood. Surprisingly, the frequency of CMV specific CD8+ T cells increased up to 30% of CD8+ T-cells at day 27 after transplantation. IFN-gamma enzyme-linked immunospot assay showed the recipient-T cells had strong responses to the A2-specific NLVPMVATV peptide. Although the phenotypic pattern of the CMV-specific T cells of the recipient was different from those of the donor before transplantation, the phenotype of the donor-derived cells retained their original phenotype in the recipient after transplantation. These finding suggested that active transferred immunity from the graft with a high frequency of CMV-specific CTL could induce a rapid reconstitution of CMV-specific T-cell mediated immunity in pediatric HLA-identical allogenetic bone marrow transplantation. The screening of peripheral blood using HLA-peptide tetramer staining might be beneficial to select donors. |
| first_indexed | 2024-03-07T00:46:23Z |
| format | Journal article |
| id | oxford-uuid:84ce0d25-adee-4201-bbdd-1ca97e4aa09d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:46:23Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:84ce0d25-adee-4201-bbdd-1ca97e4aa09d2022-03-26T21:53:24ZBone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:84ce0d25-adee-4201-bbdd-1ca97e4aa09dEnglishSymplectic Elements at Oxford2006Komatsu, HKogawa, KNonoyama, SInui, ASogo, TFujisawa, TKlenerman, PA recent study reported that quantitation of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in the graft and monitoring of these T cells might identify hematopoietic stem cell transplantation-recipients at the risk for progressive CMV infection. A 6-year-old girl underwent bone marrow transplantation from an HLA-identical sibling with a very high frequency of CMV specific tetramer-positive CD8+ T-cells. CMV-specific T-cell immunity was prospectively evaluated using a peptide (HLA-A2, NLVPMVATV). Tetramer assay showed that the frequency of CMV-specific CD8+ T cells of the donor in the peripheral blood was 5.3%, higher than average amongst young children. The frequency of CMV-specific CD8+ T cells of the donor in the graft was 3.7% of CD8+ T-cells. Before transplantation, the frequency of CMV specific CD8+ T cells of the recipient was 0.1% in the peripheral blood. Surprisingly, the frequency of CMV specific CD8+ T cells increased up to 30% of CD8+ T-cells at day 27 after transplantation. IFN-gamma enzyme-linked immunospot assay showed the recipient-T cells had strong responses to the A2-specific NLVPMVATV peptide. Although the phenotypic pattern of the CMV-specific T cells of the recipient was different from those of the donor before transplantation, the phenotype of the donor-derived cells retained their original phenotype in the recipient after transplantation. These finding suggested that active transferred immunity from the graft with a high frequency of CMV-specific CTL could induce a rapid reconstitution of CMV-specific T-cell mediated immunity in pediatric HLA-identical allogenetic bone marrow transplantation. The screening of peripheral blood using HLA-peptide tetramer staining might be beneficial to select donors. |
| spellingShingle | Komatsu, H Kogawa, K Nonoyama, S Inui, A Sogo, T Fujisawa, T Klenerman, P Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title | Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title_full | Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title_fullStr | Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title_full_unstemmed | Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title_short | Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus-specific T-cells. |
| title_sort | bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus specific t cells |
| work_keys_str_mv | AT komatsuh bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT kogawak bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT nonoyamas bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT inuia bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT sogot bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT fujisawat bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells AT klenermanp bonemarrowtransplantationfromapediatricdonorwithahighfrequencyofcytomegalovirusspecifictcells |