Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells
Attachment of the human tissue plasminogen activator signal peptide (tPA-SP) to a variety of proteins has enhanced the induction of both antibody and cellular immune responses in animal models. Here, as part of a continuing effort to improve HIV-1 vaccine immunogenicity, we sought to determine wheth...
| Main Authors: | , , , , |
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| Format: | Journal article |
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Sci Forschen
2016
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| _version_ | 1797079865353043968 |
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| author | Hanke, T Ondondo, B Abdul-Jawad, S Roshorm, Y Bridgeman, A |
| author_facet | Hanke, T Ondondo, B Abdul-Jawad, S Roshorm, Y Bridgeman, A |
| author_sort | Hanke, T |
| collection | OXFORD |
| description | Attachment of the human tissue plasminogen activator signal peptide (tPA-SP) to a variety of proteins has enhanced the induction of both antibody and cellular immune responses in animal models. Here, as part of a continuing effort to improve HIV-1 vaccine immunogenicity, we sought to determine whether or not addition of optimized tPA-SP improves the magnitude and functionality of T cells elicited by vaccines expressing protein HIVconsv derived from highly conserved regions of the HIV-1 proteome. The HIVconsv vaccines were vectored by plasmid DNA, non-replicating simian (chimpanzee) adenovirus ChAdV63 and non-replicating poxvirus MVA. We found that addition of the tPA-SP increased levels of intracellular HIVconsv protein for all the tested vaccines. When administered intramuscularly to the BALB/c mice, a 2- to 3.5-fold increase in frequencies of vaccine-elicited T-cells were observed for the DNA modality, however, there was no consistent significant improvement when using a much more potent heterologous regimen of ChAdV63 prime-MVA boost. Our results concur with previous DNA studies and emphasize that immunopotentiation by the tPA-SP is likely immunogen, vaccine modality, type of immune responses and species specific. Thus, the tPA-SP effect on induction of desired immune responses should be assessed for each vaccine strategy separately. |
| first_indexed | 2024-03-07T00:51:56Z |
| format | Journal article |
| id | oxford-uuid:86b3a2ab-2c7d-403e-98ce-5717d5c97926 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T00:51:56Z |
| publishDate | 2016 |
| publisher | Sci Forschen |
| record_format | dspace |
| spelling | oxford-uuid:86b3a2ab-2c7d-403e-98ce-5717d5c979262022-03-26T22:05:45ZVector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:86b3a2ab-2c7d-403e-98ce-5717d5c97926Symplectic Elements at OxfordSci Forschen2016Hanke, TOndondo, BAbdul-Jawad, SRoshorm, YBridgeman, AAttachment of the human tissue plasminogen activator signal peptide (tPA-SP) to a variety of proteins has enhanced the induction of both antibody and cellular immune responses in animal models. Here, as part of a continuing effort to improve HIV-1 vaccine immunogenicity, we sought to determine whether or not addition of optimized tPA-SP improves the magnitude and functionality of T cells elicited by vaccines expressing protein HIVconsv derived from highly conserved regions of the HIV-1 proteome. The HIVconsv vaccines were vectored by plasmid DNA, non-replicating simian (chimpanzee) adenovirus ChAdV63 and non-replicating poxvirus MVA. We found that addition of the tPA-SP increased levels of intracellular HIVconsv protein for all the tested vaccines. When administered intramuscularly to the BALB/c mice, a 2- to 3.5-fold increase in frequencies of vaccine-elicited T-cells were observed for the DNA modality, however, there was no consistent significant improvement when using a much more potent heterologous regimen of ChAdV63 prime-MVA boost. Our results concur with previous DNA studies and emphasize that immunopotentiation by the tPA-SP is likely immunogen, vaccine modality, type of immune responses and species specific. Thus, the tPA-SP effect on induction of desired immune responses should be assessed for each vaccine strategy separately. |
| spellingShingle | Hanke, T Ondondo, B Abdul-Jawad, S Roshorm, Y Bridgeman, A Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title | Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title_full | Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title_fullStr | Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title_full_unstemmed | Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title_short | Vector delivery-dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of T cells |
| title_sort | vector delivery dependant effect of human tissue plasminogen activator signal peptide on vaccine induction of t cells |
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