Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes.
Quantitative morphometry of immunostained pancreatic islet cells in a surgical specimen from a maturity-onset diabetic (MOD) patient (A) has been combined with a study of beta-cell secretion in him and his MOD son (B). The morphometry showed intra-islet amyloid deposits in 24% of islet sections whic...
| المؤلفون الرئيسيون: | , , , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
1987
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| _version_ | 1826283035330347008 |
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| author | Clark, A Matthews, DR Naylor, B Wells, C Hosker, J Turner, R |
| author_facet | Clark, A Matthews, DR Naylor, B Wells, C Hosker, J Turner, R |
| author_sort | Clark, A |
| collection | OXFORD |
| description | Quantitative morphometry of immunostained pancreatic islet cells in a surgical specimen from a maturity-onset diabetic (MOD) patient (A) has been combined with a study of beta-cell secretion in him and his MOD son (B). The morphometry showed intra-islet amyloid deposits in 24% of islet sections which is a similar distribution to that found in 7 other MODs, (median 24%, range 0-95%). The distribution of islet cells in the pancreas from A was similar to the 7 MODs and 9 age matched non-diabetic subjects (mean beta-cell area per exocrine area, A, 2%; MODs, 1.45%; controls 1.8%). There was a significantly smaller percentage beta-cell area per islet area in A and the other MODs compared to the controls. Beta-cell secretion was studied by an intravenous glucose infusion, 5 mg/kg ideal body wt. min-1 in A and B and 4 other MODs on sulphonylurea or diet therapy. Fasting plasma glucose was high in both A and B (16 and 7.6 mmol/l respectively) in spite of sulphonylurea therapy. Similarly, A and B had elevated fasting plasma levels of insulin, (0.055 and 0.13 pmol/ml respectively) and C-peptide (0.573 and 1.39 pmol/ml respectively). Plasma proinsulin formed a higher percentage of the immunoreactive insulin in fasting plasma samples of both A (36%) and B (43%) than in less hyperglycaemic MODs on diet alone (26%) or sulphonylurea therapy (17%) and the glucose stimulated proinsulin content was even higher (A, 50%; B, 53%; MODs, diet, 19%; sulphonylureas, 16%). Elevated proinsulin levels in a patient with islet-amyloid is consistent with the hypothesis that amyloid may be derived from abnormal beta-cell secretion. |
| first_indexed | 2024-03-07T00:52:51Z |
| format | Journal article |
| id | oxford-uuid:86fbccca-8191-41f2-a9b6-2fb36b0223fd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:52:51Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:86fbccca-8191-41f2-a9b6-2fb36b0223fd2022-03-26T22:07:49ZPancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:86fbccca-8191-41f2-a9b6-2fb36b0223fdEnglishSymplectic Elements at Oxford1987Clark, AMatthews, DRNaylor, BWells, CHosker, JTurner, RQuantitative morphometry of immunostained pancreatic islet cells in a surgical specimen from a maturity-onset diabetic (MOD) patient (A) has been combined with a study of beta-cell secretion in him and his MOD son (B). The morphometry showed intra-islet amyloid deposits in 24% of islet sections which is a similar distribution to that found in 7 other MODs, (median 24%, range 0-95%). The distribution of islet cells in the pancreas from A was similar to the 7 MODs and 9 age matched non-diabetic subjects (mean beta-cell area per exocrine area, A, 2%; MODs, 1.45%; controls 1.8%). There was a significantly smaller percentage beta-cell area per islet area in A and the other MODs compared to the controls. Beta-cell secretion was studied by an intravenous glucose infusion, 5 mg/kg ideal body wt. min-1 in A and B and 4 other MODs on sulphonylurea or diet therapy. Fasting plasma glucose was high in both A and B (16 and 7.6 mmol/l respectively) in spite of sulphonylurea therapy. Similarly, A and B had elevated fasting plasma levels of insulin, (0.055 and 0.13 pmol/ml respectively) and C-peptide (0.573 and 1.39 pmol/ml respectively). Plasma proinsulin formed a higher percentage of the immunoreactive insulin in fasting plasma samples of both A (36%) and B (43%) than in less hyperglycaemic MODs on diet alone (26%) or sulphonylurea therapy (17%) and the glucose stimulated proinsulin content was even higher (A, 50%; B, 53%; MODs, diet, 19%; sulphonylureas, 16%). Elevated proinsulin levels in a patient with islet-amyloid is consistent with the hypothesis that amyloid may be derived from abnormal beta-cell secretion. |
| spellingShingle | Clark, A Matthews, DR Naylor, B Wells, C Hosker, J Turner, R Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title | Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title_full | Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title_fullStr | Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title_full_unstemmed | Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title_short | Pancreatic islet amyloid and elevated proinsulin secretion in familial maturity-onset diabetes. |
| title_sort | pancreatic islet amyloid and elevated proinsulin secretion in familial maturity onset diabetes |
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