A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease.
Many human T-cell responses specific for epitopes in Plasmodium falciparum have been described, but none has yet been shown to be predictive of protection against natural malaria infection. Here we report a peptide-specific T-cell assay that is strongly associated with protection of humans in The Ga...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1797079944510046208 |
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| author | Reece, W Pinder, M Gothard, P Milligan, P Bojang, K Doherty, T Plebanski, M Akinwunmi, P Everaere, S Watkins, K Voss, G Tornieporth, N Alloueche, A Greenwood, B Kester, K McAdam, K Cohen, J Hill, A |
| author_facet | Reece, W Pinder, M Gothard, P Milligan, P Bojang, K Doherty, T Plebanski, M Akinwunmi, P Everaere, S Watkins, K Voss, G Tornieporth, N Alloueche, A Greenwood, B Kester, K McAdam, K Cohen, J Hill, A |
| author_sort | Reece, W |
| collection | OXFORD |
| description | Many human T-cell responses specific for epitopes in Plasmodium falciparum have been described, but none has yet been shown to be predictive of protection against natural malaria infection. Here we report a peptide-specific T-cell assay that is strongly associated with protection of humans in The Gambia, West Africa, from both malaria infection and disease. The assay detects interferon-gamma-secreting CD4(+) T cells specific for a conserved sequence from the circumsporozoite protein, which binds to many human leukocyte antigen (HLA)-DR types. The correlation was observed using a cultured, rather than an ex vivo, ELISPOT assay that measures central memory-'type T cells rather than activated effector T cells. These findings provide direct evidence for a protective role for CD4(+) T cells in humans, and a precise target for the design of improved vaccines against P. falciparum. |
| first_indexed | 2024-03-07T00:53:07Z |
| format | Journal article |
| id | oxford-uuid:87145657-feb3-42c2-bd8f-5a58dd0df2d6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:53:07Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:87145657-feb3-42c2-bd8f-5a58dd0df2d62022-03-26T22:08:24ZA CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:87145657-feb3-42c2-bd8f-5a58dd0df2d6EnglishSymplectic Elements at Oxford2004Reece, WPinder, MGothard, PMilligan, PBojang, KDoherty, TPlebanski, MAkinwunmi, PEveraere, SWatkins, KVoss, GTornieporth, NAlloueche, AGreenwood, BKester, KMcAdam, KCohen, JHill, AMany human T-cell responses specific for epitopes in Plasmodium falciparum have been described, but none has yet been shown to be predictive of protection against natural malaria infection. Here we report a peptide-specific T-cell assay that is strongly associated with protection of humans in The Gambia, West Africa, from both malaria infection and disease. The assay detects interferon-gamma-secreting CD4(+) T cells specific for a conserved sequence from the circumsporozoite protein, which binds to many human leukocyte antigen (HLA)-DR types. The correlation was observed using a cultured, rather than an ex vivo, ELISPOT assay that measures central memory-'type T cells rather than activated effector T cells. These findings provide direct evidence for a protective role for CD4(+) T cells in humans, and a precise target for the design of improved vaccines against P. falciparum. |
| spellingShingle | Reece, W Pinder, M Gothard, P Milligan, P Bojang, K Doherty, T Plebanski, M Akinwunmi, P Everaere, S Watkins, K Voss, G Tornieporth, N Alloueche, A Greenwood, B Kester, K McAdam, K Cohen, J Hill, A A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title | A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title_full | A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title_fullStr | A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title_full_unstemmed | A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title_short | A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease. |
| title_sort | cd4 t cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural plasmodium falciparum infection and disease |
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