Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.
CD4 Th cells producing the proinflammatory cytokine IL-17 (Th17) have been implicated in a number of inflammatory arthritides including the spondyloarthritides. Th17 development is promoted by IL-23. Ankylosing spondylitis, the most common spondyloarthritis (SpA), is genetically associated with both...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2011
|
| _version_ | 1826283078354468864 |
|---|---|
| author | Bowness, P Ridley, A Shaw, J Chan, A Wong-Baeza, I Fleming, M Cummings, F McMichael, A Kollnberger, S |
| author_facet | Bowness, P Ridley, A Shaw, J Chan, A Wong-Baeza, I Fleming, M Cummings, F McMichael, A Kollnberger, S |
| author_sort | Bowness, P |
| collection | OXFORD |
| description | CD4 Th cells producing the proinflammatory cytokine IL-17 (Th17) have been implicated in a number of inflammatory arthritides including the spondyloarthritides. Th17 development is promoted by IL-23. Ankylosing spondylitis, the most common spondyloarthritis (SpA), is genetically associated with both HLA-B27 (B27) and IL-23R polymorphisms; however, the link remains unexplained. We have previously shown that B27 can form H chain dimers (termed B27(2)), which, unlike classical HLA-B27, bind the killer-cell Ig-like receptor KIR3DL2. In this article, we show that B27(2)-expressing APCs stimulate the survival, proliferation, and IL-17 production of KIR3DL2(+) CD4 T cells. KIR3DL2(+) CD4 T cells are expanded and enriched for IL-17 production in the blood and synovial fluid of patients with SpA. Despite KIR3DL2(+) cells comprising a mean of just 15% of CD4 T in the peripheral blood of SpA patients, this subset accounted for 70% of the observed increase in Th17 numbers in SpA patients compared with control subjects. TCR-stimulated peripheral blood KIR3DL2(+) CD4 T cell lines from SpA patients secreted 4-fold more IL-17 than KIR3DL2(+) lines from controls or KIR3DL2(-) CD4 T cells. Strikingly, KIR3DL2(+) CD4 T cells account for the majority of peripheral blood CD4 T cell IL-23R expression and produce more IL-17 in the presence of IL-23. Our findings link HLA-B27 with IL-17 production and suggest new therapeutic strategies in ankylosing spondylitis/SpA. |
| first_indexed | 2024-03-07T00:53:30Z |
| format | Journal article |
| id | oxford-uuid:87341329-a291-4d63-af53-1d9a8070a5e6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:53:30Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:87341329-a291-4d63-af53-1d9a8070a5e62022-03-26T22:09:11ZTh17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:87341329-a291-4d63-af53-1d9a8070a5e6EnglishSymplectic Elements at Oxford2011Bowness, PRidley, AShaw, JChan, AWong-Baeza, IFleming, MCummings, FMcMichael, AKollnberger, SCD4 Th cells producing the proinflammatory cytokine IL-17 (Th17) have been implicated in a number of inflammatory arthritides including the spondyloarthritides. Th17 development is promoted by IL-23. Ankylosing spondylitis, the most common spondyloarthritis (SpA), is genetically associated with both HLA-B27 (B27) and IL-23R polymorphisms; however, the link remains unexplained. We have previously shown that B27 can form H chain dimers (termed B27(2)), which, unlike classical HLA-B27, bind the killer-cell Ig-like receptor KIR3DL2. In this article, we show that B27(2)-expressing APCs stimulate the survival, proliferation, and IL-17 production of KIR3DL2(+) CD4 T cells. KIR3DL2(+) CD4 T cells are expanded and enriched for IL-17 production in the blood and synovial fluid of patients with SpA. Despite KIR3DL2(+) cells comprising a mean of just 15% of CD4 T in the peripheral blood of SpA patients, this subset accounted for 70% of the observed increase in Th17 numbers in SpA patients compared with control subjects. TCR-stimulated peripheral blood KIR3DL2(+) CD4 T cell lines from SpA patients secreted 4-fold more IL-17 than KIR3DL2(+) lines from controls or KIR3DL2(-) CD4 T cells. Strikingly, KIR3DL2(+) CD4 T cells account for the majority of peripheral blood CD4 T cell IL-23R expression and produce more IL-17 in the presence of IL-23. Our findings link HLA-B27 with IL-17 production and suggest new therapeutic strategies in ankylosing spondylitis/SpA. |
| spellingShingle | Bowness, P Ridley, A Shaw, J Chan, A Wong-Baeza, I Fleming, M Cummings, F McMichael, A Kollnberger, S Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title | Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title_full | Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title_fullStr | Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title_full_unstemmed | Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title_short | Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis. |
| title_sort | th17 cells expressing kir3dl2 and responsive to hla b27 homodimers are increased in ankylosing spondylitis |
| work_keys_str_mv | AT bownessp th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT ridleya th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT shawj th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT chana th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT wongbaezai th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT flemingm th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT cummingsf th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT mcmichaela th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis AT kollnbergers th17cellsexpressingkir3dl2andresponsivetohlab27homodimersareincreasedinankylosingspondylitis |