The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'.
The properties of a cpYFP [circularly permuted YFP (yellow fluorescent protein)] reported to act as a superoxide sensor have been re-examined in Arabidopsis mitochondria. We have found that the probe has high pH sensitivity and that dynamics in the cpYFP signal disappeared when the matrix pH was cla...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2011
|
| _version_ | 1797079987506905088 |
|---|---|
| author | Schwarzländer, M Logan, D Fricker, M Sweetlove, L |
| author_facet | Schwarzländer, M Logan, D Fricker, M Sweetlove, L |
| author_sort | Schwarzländer, M |
| collection | OXFORD |
| description | The properties of a cpYFP [circularly permuted YFP (yellow fluorescent protein)] reported to act as a superoxide sensor have been re-examined in Arabidopsis mitochondria. We have found that the probe has high pH sensitivity and that dynamics in the cpYFP signal disappeared when the matrix pH was clamped by nigericin. In contrast, genetic and pharmacological manipulation of matrix superoxide had no detectable effect on the cpYFP signal. These findings question the existence of superoxide flashes in mitochondria. |
| first_indexed | 2024-03-07T00:53:46Z |
| format | Journal article |
| id | oxford-uuid:874d54ab-59d1-4352-aa7f-06a015a36371 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:53:46Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:874d54ab-59d1-4352-aa7f-06a015a363712022-03-26T22:09:49ZThe circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:874d54ab-59d1-4352-aa7f-06a015a36371EnglishSymplectic Elements at Oxford2011Schwarzländer, MLogan, DFricker, MSweetlove, LThe properties of a cpYFP [circularly permuted YFP (yellow fluorescent protein)] reported to act as a superoxide sensor have been re-examined in Arabidopsis mitochondria. We have found that the probe has high pH sensitivity and that dynamics in the cpYFP signal disappeared when the matrix pH was clamped by nigericin. In contrast, genetic and pharmacological manipulation of matrix superoxide had no detectable effect on the cpYFP signal. These findings question the existence of superoxide flashes in mitochondria. |
| spellingShingle | Schwarzländer, M Logan, D Fricker, M Sweetlove, L The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title | The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title_full | The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title_fullStr | The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title_full_unstemmed | The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title_short | The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: implications for the existence of superoxide 'flashes'. |
| title_sort | circularly permuted yellow fluorescent protein cpyfp that has been used as a superoxide probe is highly responsive to ph but not superoxide in mitochondria implications for the existence of superoxide flashes |
| work_keys_str_mv | AT schwarzlanderm thecircularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT logand thecircularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT frickerm thecircularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT sweetlovel thecircularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT schwarzlanderm circularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT logand circularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT frickerm circularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes AT sweetlovel circularlypermutedyellowfluorescentproteincpyfpthathasbeenusedasasuperoxideprobeishighlyresponsivetophbutnotsuperoxideinmitochondriaimplicationsfortheexistenceofsuperoxideflashes |