HLA-A2 subtypes are functionally distinct in peptide binding and presentation.

Nearly half of HLA-A2-positive individuals in African populations have a subtype of HLA-A2 other than the A*0201 allele. We have isolated the common African HLA-A2 subtype genes from Epstein-Barr virus-transformed B cell lines and have established stable class I reduced transfectants expressing thes...

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Hoofdauteurs: Barouch, D, Friede, T, Stevanović, S, Tussey, L, Smith, K, Rowland-Jones, S, Braud, V, McMichael, A, Rammensee, H
Formaat: Journal article
Taal:English
Gepubliceerd in: 1995
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author Barouch, D
Friede, T
Stevanović, S
Tussey, L
Smith, K
Rowland-Jones, S
Braud, V
McMichael, A
Rammensee, H
author_facet Barouch, D
Friede, T
Stevanović, S
Tussey, L
Smith, K
Rowland-Jones, S
Braud, V
McMichael, A
Rammensee, H
author_sort Barouch, D
collection OXFORD
description Nearly half of HLA-A2-positive individuals in African populations have a subtype of HLA-A2 other than the A*0201 allele. We have isolated the common African HLA-A2 subtype genes from Epstein-Barr virus-transformed B cell lines and have established stable class I reduced transfectants expressing these alleles. We have studied the peptide binding and presentation properties of A*0201, A*0202, A*0205, A*0214, and A*6901 by a combination of approaches: assaying direct binding of labeled synthetic peptides, studying the ability of antigen-specific cytotoxic T lymphocytes to recognize peptide-pulsed cells, and sequencing peptide pools and individual ligands eluted from cells. We find that A*0201-restricted peptides can also bind to A*0202 but do not bind strongly to the other alleles in this study. We show that some cytotoxic T lymphocytes can recognize all subtypes capable of binding an antigenic peptide, whereas others are subtype specific. Sequencing of eluted peptides reveals that A*0202 has a similar peptide motif to A*0201, but that A*0205, A*0214, and A*6901 have different motifs. These data strongly support a model in which residue 9 (Phe or Tyr) of the A2/A68/A69 molecules is a critical factor in determining the specificity of the B pocket of the major histocompatibility complex and the position 2 anchor residue of associated peptides. We conclude that a single-amino acid difference in the major histocompatibility complex can be sufficient to cause a dramatic change in the nature of bound peptides, implying that individuals with closely related HLA subtypes may present very different repertoires of antigenic peptides to T cells in an immune response. It is likely to be a general phenomenon that very similar class I subtypes will behave as functionally distinct HLA allotypes.
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spelling oxford-uuid:87553ea7-78c0-4b2e-aa72-c0792a920c0c2022-03-26T22:10:05ZHLA-A2 subtypes are functionally distinct in peptide binding and presentation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:87553ea7-78c0-4b2e-aa72-c0792a920c0cEnglishSymplectic Elements at Oxford1995Barouch, DFriede, TStevanović, STussey, LSmith, KRowland-Jones, SBraud, VMcMichael, ARammensee, HNearly half of HLA-A2-positive individuals in African populations have a subtype of HLA-A2 other than the A*0201 allele. We have isolated the common African HLA-A2 subtype genes from Epstein-Barr virus-transformed B cell lines and have established stable class I reduced transfectants expressing these alleles. We have studied the peptide binding and presentation properties of A*0201, A*0202, A*0205, A*0214, and A*6901 by a combination of approaches: assaying direct binding of labeled synthetic peptides, studying the ability of antigen-specific cytotoxic T lymphocytes to recognize peptide-pulsed cells, and sequencing peptide pools and individual ligands eluted from cells. We find that A*0201-restricted peptides can also bind to A*0202 but do not bind strongly to the other alleles in this study. We show that some cytotoxic T lymphocytes can recognize all subtypes capable of binding an antigenic peptide, whereas others are subtype specific. Sequencing of eluted peptides reveals that A*0202 has a similar peptide motif to A*0201, but that A*0205, A*0214, and A*6901 have different motifs. These data strongly support a model in which residue 9 (Phe or Tyr) of the A2/A68/A69 molecules is a critical factor in determining the specificity of the B pocket of the major histocompatibility complex and the position 2 anchor residue of associated peptides. We conclude that a single-amino acid difference in the major histocompatibility complex can be sufficient to cause a dramatic change in the nature of bound peptides, implying that individuals with closely related HLA subtypes may present very different repertoires of antigenic peptides to T cells in an immune response. It is likely to be a general phenomenon that very similar class I subtypes will behave as functionally distinct HLA allotypes.
spellingShingle Barouch, D
Friede, T
Stevanović, S
Tussey, L
Smith, K
Rowland-Jones, S
Braud, V
McMichael, A
Rammensee, H
HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title_full HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title_fullStr HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title_full_unstemmed HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title_short HLA-A2 subtypes are functionally distinct in peptide binding and presentation.
title_sort hla a2 subtypes are functionally distinct in peptide binding and presentation
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