Anatomy and dynamics of a supramolecular membrane protein cluster.
Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescence recovery after photobleaching (FRAP) analysis, and simulations t...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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| _version_ | 1797080126382407680 |
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| author | Sieber, J Willig, K Kutzner, C Gerding-Reimers, C Harke, B Donnert, G Rammner, B Eggeling, C Hell, S Grubmüller, H Lang, T |
| author_facet | Sieber, J Willig, K Kutzner, C Gerding-Reimers, C Harke, B Donnert, G Rammner, B Eggeling, C Hell, S Grubmüller, H Lang, T |
| author_sort | Sieber, J |
| collection | OXFORD |
| description | Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescence recovery after photobleaching (FRAP) analysis, and simulations to show that clustering can be explained by self-organization based on simple physical principles. On average, the syntaxin clusters exhibit a diameter of 50 to 60 nanometers and contain 75 densely crowded syntaxins that dynamically exchange with freely diffusing molecules. Self-association depends on weak homophilic protein-protein interactions. Simulations suggest that clustering immobilizes and conformationally constrains the molecules. Moreover, a balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures. |
| first_indexed | 2024-03-07T00:55:42Z |
| format | Journal article |
| id | oxford-uuid:8801f9a7-9483-4e22-9b0b-e12f812aca86 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:55:42Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:8801f9a7-9483-4e22-9b0b-e12f812aca862022-03-26T22:14:17ZAnatomy and dynamics of a supramolecular membrane protein cluster.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8801f9a7-9483-4e22-9b0b-e12f812aca86EnglishSymplectic Elements at Oxford2007Sieber, JWillig, KKutzner, CGerding-Reimers, CHarke, BDonnert, GRammner, BEggeling, CHell, SGrubmüller, HLang, TMost plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescence recovery after photobleaching (FRAP) analysis, and simulations to show that clustering can be explained by self-organization based on simple physical principles. On average, the syntaxin clusters exhibit a diameter of 50 to 60 nanometers and contain 75 densely crowded syntaxins that dynamically exchange with freely diffusing molecules. Self-association depends on weak homophilic protein-protein interactions. Simulations suggest that clustering immobilizes and conformationally constrains the molecules. Moreover, a balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures. |
| spellingShingle | Sieber, J Willig, K Kutzner, C Gerding-Reimers, C Harke, B Donnert, G Rammner, B Eggeling, C Hell, S Grubmüller, H Lang, T Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title | Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title_full | Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title_fullStr | Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title_full_unstemmed | Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title_short | Anatomy and dynamics of a supramolecular membrane protein cluster. |
| title_sort | anatomy and dynamics of a supramolecular membrane protein cluster |
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