STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C
Reaction of a 1:1 mixture of penicillin N and 2,2-bis(trideuteriomethyl) penicillin N with partially purified deacetoxy-cephalosporin C/deacetylcephalosporin C synthetase from Cephalosporium acremonium CO 728 resulted in preferential conversion of the protiated substrate, whereas in similar experime...
| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
1987
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| _version_ | 1826283245450297344 |
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| author | Baldwin, J Adlington, R Aplin, R Crouch, N Schofield, C Ting, H |
| author_facet | Baldwin, J Adlington, R Aplin, R Crouch, N Schofield, C Ting, H |
| author_sort | Baldwin, J |
| collection | OXFORD |
| description | Reaction of a 1:1 mixture of penicillin N and 2,2-bis(trideuteriomethyl) penicillin N with partially purified deacetoxy-cephalosporin C/deacetylcephalosporin C synthetase from Cephalosporium acremonium CO 728 resulted in preferential conversion of the protiated substrate, whereas in similar experiments with a 1:1 mixture of penicillin N and 3-deuteriopenicillin N both substrates were converted at the same rate; these results accord with a stepwise process for hydrogen removal during the ring expansion in which the methyl hydrogen is first removed. |
| first_indexed | 2024-03-07T00:56:00Z |
| format | Journal article |
| id | oxford-uuid:881c773b-1311-40b5-865c-e280dce6029f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:56:00Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:881c773b-1311-40b5-865c-e280dce6029f2022-03-26T22:14:56ZSTEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-CJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:881c773b-1311-40b5-865c-e280dce6029fEnglishSymplectic Elements at Oxford1987Baldwin, JAdlington, RAplin, RCrouch, NSchofield, CTing, HReaction of a 1:1 mixture of penicillin N and 2,2-bis(trideuteriomethyl) penicillin N with partially purified deacetoxy-cephalosporin C/deacetylcephalosporin C synthetase from Cephalosporium acremonium CO 728 resulted in preferential conversion of the protiated substrate, whereas in similar experiments with a 1:1 mixture of penicillin N and 3-deuteriopenicillin N both substrates were converted at the same rate; these results accord with a stepwise process for hydrogen removal during the ring expansion in which the methyl hydrogen is first removed. |
| spellingShingle | Baldwin, J Adlington, R Aplin, R Crouch, N Schofield, C Ting, H STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title | STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title_full | STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title_fullStr | STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title_full_unstemmed | STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title_short | STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C |
| title_sort | stepwise hydrogen removal in the enzymatic ring expansion of penicillin n to deacetoxycephalosporin c |
| work_keys_str_mv | AT baldwinj stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc AT adlingtonr stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc AT aplinr stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc AT crouchn stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc AT schofieldc stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc AT tingh stepwisehydrogenremovalintheenzymaticringexpansionofpenicillinntodeacetoxycephalosporinc |