Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26
The interaction between the reduced primary acceptor quinone (Q(A)-.), and the triplet state of the primary donor 3D is investigated with time-resolved continuous-wave EPR. The trough at high-field in the Q(A)-. electron-spin polarized X-band EPR-spectrum at early delay times after the laser flash [...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1996
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| _version_ | 1797080162488025088 |
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| author | Bosch, M Gast, P Franken, E Zwanenburg, G Hore, P Hoff, A |
| author_facet | Bosch, M Gast, P Franken, E Zwanenburg, G Hore, P Hoff, A |
| author_sort | Bosch, M |
| collection | OXFORD |
| description | The interaction between the reduced primary acceptor quinone (Q(A)-.), and the triplet state of the primary donor 3D is investigated with time-resolved continuous-wave EPR. The trough at high-field in the Q(A)-. electron-spin polarized X-band EPR-spectrum at early delay times after the laser flash [De Groot et al. (1985) Biochim. Biophys. Acta 808, 13-20] is studied as a function of temperature and of the delay, in zinc-reconstituted reaction centers, with and without carotenoid. In all cases investigated a decrease in 3D concentration is accompanied by a simultaneous attenuation of the high-field trough in the Q(A)-. EPR spectrum. These observations confirm the hypothesis that the line-shape of Q(A)-. at short delay times is influenced by a magnetic interaction with 3D. The line-shape of the Q(A)-. electron-spin polarized EPR spectrum directly after the laser flash, could be very well simulated using an extension of the model of Here et al. [Hore, P.J. et al. (1993) Biochim. Biophys. Acta 1141, 221-230], with dipolar coupling between Q(A)-. and 3D of -0.125 mT. |
| first_indexed | 2024-03-07T00:56:15Z |
| format | Journal article |
| id | oxford-uuid:88305b11-8c92-42a1-b907-eabe2a83c300 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:56:15Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:88305b11-8c92-42a1-b907-eabe2a83c3002022-03-26T22:15:22ZMagnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:88305b11-8c92-42a1-b907-eabe2a83c300EnglishSymplectic Elements at Oxford1996Bosch, MGast, PFranken, EZwanenburg, GHore, PHoff, AThe interaction between the reduced primary acceptor quinone (Q(A)-.), and the triplet state of the primary donor 3D is investigated with time-resolved continuous-wave EPR. The trough at high-field in the Q(A)-. electron-spin polarized X-band EPR-spectrum at early delay times after the laser flash [De Groot et al. (1985) Biochim. Biophys. Acta 808, 13-20] is studied as a function of temperature and of the delay, in zinc-reconstituted reaction centers, with and without carotenoid. In all cases investigated a decrease in 3D concentration is accompanied by a simultaneous attenuation of the high-field trough in the Q(A)-. EPR spectrum. These observations confirm the hypothesis that the line-shape of Q(A)-. at short delay times is influenced by a magnetic interaction with 3D. The line-shape of the Q(A)-. electron-spin polarized EPR spectrum directly after the laser flash, could be very well simulated using an extension of the model of Here et al. [Hore, P.J. et al. (1993) Biochim. Biophys. Acta 1141, 221-230], with dipolar coupling between Q(A)-. and 3D of -0.125 mT. |
| spellingShingle | Bosch, M Gast, P Franken, E Zwanenburg, G Hore, P Hoff, A Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title | Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title_full | Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title_fullStr | Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title_full_unstemmed | Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title_short | Magnetic interaction between Q(A)(-center dot) and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium Rhodobacter sphaeroides R26 |
| title_sort | magnetic interaction between q a center dot and the triplet state of the primary donor in modified reaction centers of the photosynthetic bacterium rhodobacter sphaeroides r26 |
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