Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept
Molecular epidemiological data are key for dengue outbreak characterization and preparedness. However, sparse Dengue virus (DENV) molecular information is available in Laos because of limited resources. In this proof-of-concept study, we evaluated whether DENV1 RNA extracted from rapid diagnostic te...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
American Society of Tropical Medicine and Hygiene
2019
|
| _version_ | 1797080319619235840 |
|---|---|
| author | Cassidy-Seyoum, S Vongsouvath, M Sengvilaipaseuth, O Seephonelee, M Bharucha, T De Lamballerie, X Newton, P Dubot-Pérès, A |
| author_facet | Cassidy-Seyoum, S Vongsouvath, M Sengvilaipaseuth, O Seephonelee, M Bharucha, T De Lamballerie, X Newton, P Dubot-Pérès, A |
| author_sort | Cassidy-Seyoum, S |
| collection | OXFORD |
| description | Molecular epidemiological data are key for dengue outbreak characterization and preparedness. However, sparse Dengue virus (DENV) molecular information is available in Laos because of limited resources. In this proof-of-concept study, we evaluated whether DENV1 RNA extracted from rapid diagnostic tests (RDTs) could be amplified and sequenced. The protocol for envelope gene amplification from RNA purified from RDTs was first assessed using viral isolate dilutions then conducted using 14 dengue patient sera. Envelope gene amplification was successful from patient sera with high virus titer, as was sequencing but with lower efficiency. Hence, based on our results, RDTs can be a source of DENV1 RNA for subsequent envelope gene amplification and sequencing. This is a promising tool for collecting molecular epidemiology data from rural dengue-endemic areas. However, further investigations are needed to improve assay efficiency and to assess this tool's level of efficacy on a larger scale in the field. |
| first_indexed | 2024-03-07T00:58:17Z |
| format | Journal article |
| id | oxford-uuid:88d70a17-0383-47b9-93a3-3fe37bf6a431 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:58:17Z |
| publishDate | 2019 |
| publisher | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | oxford-uuid:88d70a17-0383-47b9-93a3-3fe37bf6a4312022-03-26T22:20:15ZRapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of conceptJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:88d70a17-0383-47b9-93a3-3fe37bf6a431EnglishSymplectic Elements at OxfordAmerican Society of Tropical Medicine and Hygiene2019Cassidy-Seyoum, SVongsouvath, MSengvilaipaseuth, OSeephonelee, MBharucha, TDe Lamballerie, XNewton, PDubot-Pérès, AMolecular epidemiological data are key for dengue outbreak characterization and preparedness. However, sparse Dengue virus (DENV) molecular information is available in Laos because of limited resources. In this proof-of-concept study, we evaluated whether DENV1 RNA extracted from rapid diagnostic tests (RDTs) could be amplified and sequenced. The protocol for envelope gene amplification from RNA purified from RDTs was first assessed using viral isolate dilutions then conducted using 14 dengue patient sera. Envelope gene amplification was successful from patient sera with high virus titer, as was sequencing but with lower efficiency. Hence, based on our results, RDTs can be a source of DENV1 RNA for subsequent envelope gene amplification and sequencing. This is a promising tool for collecting molecular epidemiology data from rural dengue-endemic areas. However, further investigations are needed to improve assay efficiency and to assess this tool's level of efficacy on a larger scale in the field. |
| spellingShingle | Cassidy-Seyoum, S Vongsouvath, M Sengvilaipaseuth, O Seephonelee, M Bharucha, T De Lamballerie, X Newton, P Dubot-Pérès, A Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title | Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title_full | Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title_fullStr | Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title_full_unstemmed | Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title_short | Rapid diagnostic tests as a source of dengue virus RNA for envelope gene amplification: a proof of concept |
| title_sort | rapid diagnostic tests as a source of dengue virus rna for envelope gene amplification a proof of concept |
| work_keys_str_mv | AT cassidyseyoums rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT vongsouvathm rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT sengvilaipaseutho rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT seephoneleem rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT bharuchat rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT delamballeriex rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT newtonp rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept AT dubotperesa rapiddiagnostictestsasasourceofdenguevirusrnaforenvelopegeneamplificationaproofofconcept |