Defining the T cell antigen proteome of wasp venom.
BACKGROUND: While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens. OBJECTIVE: We sought comprehensively to characterize the T cell proteome for...
| 主要な著者: | , , , , , , |
|---|---|
| フォーマット: | Journal article |
| 言語: | English |
| 出版事項: |
2006
|
| _version_ | 1826283496239267840 |
|---|---|
| author | Aslam, A Kessler, B Batycka, M O'Callaghan, C Misbah, SA Warrell, D Ogg, G |
| author_facet | Aslam, A Kessler, B Batycka, M O'Callaghan, C Misbah, SA Warrell, D Ogg, G |
| author_sort | Aslam, A |
| collection | OXFORD |
| description | BACKGROUND: While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens. OBJECTIVE: We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell-based immunotherapeutic approaches. METHODS: Using peripheral blood mononuclear cells from wasp venom-allergic individuals and IL-4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange-fractionated venom. Reactive fractions were purified and identified using highly sensitive electrospray ion-trap mass spectrometry. RESULTS: Wasp venom-allergic individuals have detectable whole wasp venom-specific T cells directly ex vivo, which show rapid IL-4 effector function. T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A(1), hyaluronidase and antigen 5. CONCLUSION: Although it is likely that there are many T cell antigens within wasp venom, the main responses are to proteins coincident with the known IgE-binding proteins. |
| first_indexed | 2024-03-07T00:59:45Z |
| format | Journal article |
| id | oxford-uuid:894fb488-bb2a-4527-8273-89c0d2a49ca0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T00:59:45Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:894fb488-bb2a-4527-8273-89c0d2a49ca02022-03-26T22:23:39ZDefining the T cell antigen proteome of wasp venom.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:894fb488-bb2a-4527-8273-89c0d2a49ca0EnglishSymplectic Elements at Oxford2006Aslam, AKessler, BBatycka, MO'Callaghan, CMisbah, SAWarrell, DOgg, GBACKGROUND: While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens. OBJECTIVE: We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell-based immunotherapeutic approaches. METHODS: Using peripheral blood mononuclear cells from wasp venom-allergic individuals and IL-4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange-fractionated venom. Reactive fractions were purified and identified using highly sensitive electrospray ion-trap mass spectrometry. RESULTS: Wasp venom-allergic individuals have detectable whole wasp venom-specific T cells directly ex vivo, which show rapid IL-4 effector function. T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A(1), hyaluronidase and antigen 5. CONCLUSION: Although it is likely that there are many T cell antigens within wasp venom, the main responses are to proteins coincident with the known IgE-binding proteins. |
| spellingShingle | Aslam, A Kessler, B Batycka, M O'Callaghan, C Misbah, SA Warrell, D Ogg, G Defining the T cell antigen proteome of wasp venom. |
| title | Defining the T cell antigen proteome of wasp venom. |
| title_full | Defining the T cell antigen proteome of wasp venom. |
| title_fullStr | Defining the T cell antigen proteome of wasp venom. |
| title_full_unstemmed | Defining the T cell antigen proteome of wasp venom. |
| title_short | Defining the T cell antigen proteome of wasp venom. |
| title_sort | defining the t cell antigen proteome of wasp venom |
| work_keys_str_mv | AT aslama definingthetcellantigenproteomeofwaspvenom AT kesslerb definingthetcellantigenproteomeofwaspvenom AT batyckam definingthetcellantigenproteomeofwaspvenom AT ocallaghanc definingthetcellantigenproteomeofwaspvenom AT misbahsa definingthetcellantigenproteomeofwaspvenom AT warrelld definingthetcellantigenproteomeofwaspvenom AT oggg definingthetcellantigenproteomeofwaspvenom |