Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA).
This report contains a description of the cellular localization and kinetics of proinflammatory cytokine expression in murine CIA, a model for rheumatoid arthritis. Tissue cryostat sections of undecalcified paws from type II collagen-immunized DBA/1 mice, taken 1-10 days after the onset of clinical...
| Główni autorzy: | , , , , , |
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| Format: | Journal article |
| Język: | English |
| Wydane: |
1997
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| _version_ | 1826283535244197888 |
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| author | Marinova-Mutafchieva, L Williams, R Mason, L Mauri, C Feldmann, M Maini, R |
| author_facet | Marinova-Mutafchieva, L Williams, R Mason, L Mauri, C Feldmann, M Maini, R |
| author_sort | Marinova-Mutafchieva, L |
| collection | OXFORD |
| description | This report contains a description of the cellular localization and kinetics of proinflammatory cytokine expression in murine CIA, a model for rheumatoid arthritis. Tissue cryostat sections of undecalcified paws from type II collagen-immunized DBA/1 mice, taken 1-10 days after the onset of clinical arthritis, were examined for the presence of tumour necrosis factor-alpha (TNF-alpha), IL-1beta and IL-6 using an indirect immunoperoxidase technique. In parallel, interferon-gamma (IFN-gamma) production by lymph node cells, stimulated in vitro with type II collagen, was assessed as a marker of T cell activity. The main areas of TNF-alpha, IL-1beta and IL-6 expression were in the synovial lining layer and in tissue contiguous with cartilage and bone (the marginal zone), in particular at sites of pannus formation and joint erosion. There was a progressive increase in the number of TNF-alpha-, IL-1beta- and IL-6-positive cells from day 1 to day 10 of arthritis, during which time IFN-gamma production by CD4+ T cells from draining lymph nodes declined sharply. A further finding of potential significance was that TNF-alpha was consistently detected at day 1 of arthritis, whereas IL- 1beta-positive cells were not found until day 3, suggesting that the expression of TNF-alpha precedes that of IL-1beta. |
| first_indexed | 2024-03-07T01:00:19Z |
| format | Journal article |
| id | oxford-uuid:897d330f-9aaf-44f2-9e24-bef572820c51 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:00:19Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:897d330f-9aaf-44f2-9e24-bef572820c512022-03-26T22:25:03ZDynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA).Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:897d330f-9aaf-44f2-9e24-bef572820c51EnglishSymplectic Elements at Oxford1997Marinova-Mutafchieva, LWilliams, RMason, LMauri, CFeldmann, MMaini, RThis report contains a description of the cellular localization and kinetics of proinflammatory cytokine expression in murine CIA, a model for rheumatoid arthritis. Tissue cryostat sections of undecalcified paws from type II collagen-immunized DBA/1 mice, taken 1-10 days after the onset of clinical arthritis, were examined for the presence of tumour necrosis factor-alpha (TNF-alpha), IL-1beta and IL-6 using an indirect immunoperoxidase technique. In parallel, interferon-gamma (IFN-gamma) production by lymph node cells, stimulated in vitro with type II collagen, was assessed as a marker of T cell activity. The main areas of TNF-alpha, IL-1beta and IL-6 expression were in the synovial lining layer and in tissue contiguous with cartilage and bone (the marginal zone), in particular at sites of pannus formation and joint erosion. There was a progressive increase in the number of TNF-alpha-, IL-1beta- and IL-6-positive cells from day 1 to day 10 of arthritis, during which time IFN-gamma production by CD4+ T cells from draining lymph nodes declined sharply. A further finding of potential significance was that TNF-alpha was consistently detected at day 1 of arthritis, whereas IL- 1beta-positive cells were not found until day 3, suggesting that the expression of TNF-alpha precedes that of IL-1beta. |
| spellingShingle | Marinova-Mutafchieva, L Williams, R Mason, L Mauri, C Feldmann, M Maini, R Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title | Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title_full | Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title_fullStr | Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title_full_unstemmed | Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title_short | Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). |
| title_sort | dynamics of proinflammatory cytokine expression in the joints of mice with collagen induced arthritis cia |
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