Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells.
We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has be...
| Autori principali: | , , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
1994
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| _version_ | 1826283566206550016 |
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| author | Maxwell, P Ferguson, D Osmond, M Pugh, C Heryet, A Doe, BG Johnson, M Ratcliffe, P |
| author_facet | Maxwell, P Ferguson, D Osmond, M Pugh, C Heryet, A Doe, BG Johnson, M Ratcliffe, P |
| author_sort | Maxwell, P |
| collection | OXFORD |
| description | We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney. |
| first_indexed | 2024-03-07T01:00:48Z |
| format | Journal article |
| id | oxford-uuid:89a31b07-46b8-4e33-8a27-2f72bc0bcc92 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:00:48Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:89a31b07-46b8-4e33-8a27-2f72bc0bcc922022-03-26T22:26:04ZExpression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:89a31b07-46b8-4e33-8a27-2f72bc0bcc92EnglishSymplectic Elements at Oxford1994Maxwell, PFerguson, DOsmond, MPugh, CHeryet, ADoe, BGJohnson, MRatcliffe, PWe have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney. |
| spellingShingle | Maxwell, P Ferguson, D Osmond, M Pugh, C Heryet, A Doe, BG Johnson, M Ratcliffe, P Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title | Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title_full | Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title_fullStr | Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title_full_unstemmed | Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title_short | Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. |
| title_sort | expression of a homologously recombined erythopoietin sv40 t antigen fusion gene in mouse liver evidence for erythropoietin production by ito cells |
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