The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.

Non-radiative dissipation of absorbed excitation energy in chloroplast membranes is induced in the presence of the trans-thylakoid proton motive force; this dissipation is measured as high energy state quenching of chlorophyll fluorescence, qE. It has been suggested that this results from a low pH-i...

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Main Authors: Ruban, A, Walters, R, Horton, P
Format: Journal article
Language:English
Published: 1992
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author Ruban, A
Walters, R
Horton, P
author_facet Ruban, A
Walters, R
Horton, P
author_sort Ruban, A
collection OXFORD
description Non-radiative dissipation of absorbed excitation energy in chloroplast membranes is induced in the presence of the trans-thylakoid proton motive force; this dissipation is measured as high energy state quenching of chlorophyll fluorescence, qE. It has been suggested that this results from a low pH-induced structural alteration in the light harvesting complex of photosystem II, LHCII [(1991) FEBS Letters 292, 1-4]. The effect of the carboxyl-modifying agent, dicyclohexylcarbodiimide (DCCD), on energy dissipation in chloroplast membranes has been investigated. At concentrations below that required to inhibit electron transport, DCCD caused a decrease in the steady state delta pH, completely inhibited qE and also inhibited the low pH-dependent induction of qE. DCCD binding to polypeptides in the 22-28 kDa range correlated with inhibition of qE. It is suggested that DCCD reacts with amino acid residues in LHCII whose protonation is the primary event in the induction of energy dissipation. This LHCII domain may be identical to one forming a proton channel linking the site of PSII-dependent water oxidation to the thylakoid lumen [(1990) Eur. J. Biochem. 193, 731-736].
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spelling oxford-uuid:89ebb834-33cb-432b-af4c-29751703c1a92022-03-26T22:27:53ZThe molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:89ebb834-33cb-432b-af4c-29751703c1a9EnglishSymplectic Elements at Oxford1992Ruban, AWalters, RHorton, PNon-radiative dissipation of absorbed excitation energy in chloroplast membranes is induced in the presence of the trans-thylakoid proton motive force; this dissipation is measured as high energy state quenching of chlorophyll fluorescence, qE. It has been suggested that this results from a low pH-induced structural alteration in the light harvesting complex of photosystem II, LHCII [(1991) FEBS Letters 292, 1-4]. The effect of the carboxyl-modifying agent, dicyclohexylcarbodiimide (DCCD), on energy dissipation in chloroplast membranes has been investigated. At concentrations below that required to inhibit electron transport, DCCD caused a decrease in the steady state delta pH, completely inhibited qE and also inhibited the low pH-dependent induction of qE. DCCD binding to polypeptides in the 22-28 kDa range correlated with inhibition of qE. It is suggested that DCCD reacts with amino acid residues in LHCII whose protonation is the primary event in the induction of energy dissipation. This LHCII domain may be identical to one forming a proton channel linking the site of PSII-dependent water oxidation to the thylakoid lumen [(1990) Eur. J. Biochem. 193, 731-736].
spellingShingle Ruban, A
Walters, R
Horton, P
The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title_full The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title_fullStr The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title_full_unstemmed The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title_short The molecular mechanism of the control of excitation energy dissipation in chloroplast membranes. Inhibition of delta pH-dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide.
title_sort molecular mechanism of the control of excitation energy dissipation in chloroplast membranes inhibition of delta ph dependent quenching of chlorophyll fluorescence by dicyclohexylcarbodiimide
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