Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery.
The distribution of functionally active, inwardly rectifying K (K(IR)) channels was investigated in the rat small mesenteric artery using both freshly isolated smooth muscle and endothelial cells and small arterial segments. In Ca(2+)-free solution, endothelial cells displayed a K(IR) current with a...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2003
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| _version_ | 1797080594894553088 |
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| author | Crane, G Walker, S Dora, K Garland, C |
| author_facet | Crane, G Walker, S Dora, K Garland, C |
| author_sort | Crane, G |
| collection | OXFORD |
| description | The distribution of functionally active, inwardly rectifying K (K(IR)) channels was investigated in the rat small mesenteric artery using both freshly isolated smooth muscle and endothelial cells and small arterial segments. In Ca(2+)-free solution, endothelial cells displayed a K(IR) current with a maximum amplitude of 190 +/- 16 pA at -150 mV and sensitivity to block with 30 microM Ba(2+) (n = 7). In smooth muscle cells, outward K current was activated at around -47 +/- 3 mV, but there was no evidence of K(IR) current (n = 6). Furthermore, raising extracellular [K(+)] to either 60 or 140 mM, or applying the alpha(1)-adrenoceptor agonist phenylephrine (PE; 30 microM), failed to reveal an inwardly rectifying current in the smooth muscle cells, although PE did stimulate an iberiotoxin-sensitive outward K current (n = 4). Exogenous K(+) (10.8-16.8 mM) both relaxed and repolarized endothelium-denuded segments of the mesenteric artery contracted with PE. These effects were depressed by 100 microM ouabain but unaffected by either 30 microM BaCl(2) or 3 microM glibenclamide. These data suggest that functional, inwardly rectifying Ba(2+)-sensitive channels are restricted to the endothelial cell layer in the rat small mesenteric artery. |
| first_indexed | 2024-03-07T01:02:21Z |
| format | Journal article |
| id | oxford-uuid:8a28c31f-c984-4912-8061-d1bc876bedb5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:02:21Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:8a28c31f-c984-4912-8061-d1bc876bedb52022-03-26T22:29:30ZEvidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8a28c31f-c984-4912-8061-d1bc876bedb5EnglishSymplectic Elements at Oxford2003Crane, GWalker, SDora, KGarland, CThe distribution of functionally active, inwardly rectifying K (K(IR)) channels was investigated in the rat small mesenteric artery using both freshly isolated smooth muscle and endothelial cells and small arterial segments. In Ca(2+)-free solution, endothelial cells displayed a K(IR) current with a maximum amplitude of 190 +/- 16 pA at -150 mV and sensitivity to block with 30 microM Ba(2+) (n = 7). In smooth muscle cells, outward K current was activated at around -47 +/- 3 mV, but there was no evidence of K(IR) current (n = 6). Furthermore, raising extracellular [K(+)] to either 60 or 140 mM, or applying the alpha(1)-adrenoceptor agonist phenylephrine (PE; 30 microM), failed to reveal an inwardly rectifying current in the smooth muscle cells, although PE did stimulate an iberiotoxin-sensitive outward K current (n = 4). Exogenous K(+) (10.8-16.8 mM) both relaxed and repolarized endothelium-denuded segments of the mesenteric artery contracted with PE. These effects were depressed by 100 microM ouabain but unaffected by either 30 microM BaCl(2) or 3 microM glibenclamide. These data suggest that functional, inwardly rectifying Ba(2+)-sensitive channels are restricted to the endothelial cell layer in the rat small mesenteric artery. |
| spellingShingle | Crane, G Walker, S Dora, K Garland, C Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title | Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title_full | Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title_fullStr | Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title_full_unstemmed | Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title_short | Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. |
| title_sort | evidence for a differential cellular distribution of inward rectifier k channels in the rat isolated mesenteric artery |
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