Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.
Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their abil...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1992
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| _version_ | 1797080786943344640 |
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| author | Monaco, A Lark, Z Lehrach, H |
| author_facet | Monaco, A Lark, Z Lehrach, H |
| author_sort | Monaco, A |
| collection | OXFORD |
| description | Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries. |
| first_indexed | 2024-03-07T01:05:11Z |
| format | Journal article |
| id | oxford-uuid:8b187b76-4a20-4a53-ad23-b45575911e32 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:05:11Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:8b187b76-4a20-4a53-ad23-b45575911e322022-03-26T22:35:55ZConstruction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8b187b76-4a20-4a53-ad23-b45575911e32EnglishSymplectic Elements at Oxford1992Monaco, ALark, ZLehrach, HYeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1-6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100-1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries. |
| spellingShingle | Monaco, A Lark, Z Lehrach, H Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title | Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title_full | Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title_fullStr | Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title_full_unstemmed | Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title_short | Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis. |
| title_sort | construction of yeast artificial chromosome libraries by pulsed field gel electrophoresis |
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