Comprehensive identification of RNA-binding domains in human cells
Mammalian cells harbor more than a thousand RNA-binding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified 1174 binding sites within 529 HeLa cell RBPs, discovering...
| Автори: | , , , , , , , , |
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| Формат: | Journal article |
| Опубліковано: |
Cell Press
2016
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| _version_ | 1826283921292132352 |
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| author | Castello, A Fischer, B Frese, C Horos, R Alleaume, A Foehr, S Curk, T Krijgsveld, J Hentze, M |
| author_facet | Castello, A Fischer, B Frese, C Horos, R Alleaume, A Foehr, S Curk, T Krijgsveld, J Hentze, M |
| author_sort | Castello, A |
| collection | OXFORD |
| description | Mammalian cells harbor more than a thousand RNA-binding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified 1174 binding sites within 529 HeLa cell RBPs, discovering numerous RNA-binding domains (RBDs). Catalytic centers or protein-protein interaction domains are in close relationship with RNA-binding sites, invoking possible effector roles of RNA in the control of protein function. Nearly half of the RNA-binding sites map to intrinsically disordered regions, uncovering unstructured domains as prevalent partners in protein-RNA interactions. RNA-binding sites represent hot spots for defined posttranslational modifications such as lysine acetylation and tyrosine phosphorylation, suggesting metabolic and signal-dependent regulation of RBP function. RBDs display a high degree of evolutionary conservation and incidence of Mendelian mutations, suggestive of important functional roles. RBDmap thus yields profound insights into native protein-RNA interactions in living cells. |
| first_indexed | 2024-03-07T01:06:06Z |
| format | Journal article |
| id | oxford-uuid:8b64c827-a036-435e-94e7-2f3723419e63 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:06:06Z |
| publishDate | 2016 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:8b64c827-a036-435e-94e7-2f3723419e632022-03-26T22:37:45ZComprehensive identification of RNA-binding domains in human cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8b64c827-a036-435e-94e7-2f3723419e63Symplectic Elements at OxfordCell Press2016Castello, AFischer, BFrese, CHoros, RAlleaume, AFoehr, SCurk, TKrijgsveld, JHentze, MMammalian cells harbor more than a thousand RNA-binding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified 1174 binding sites within 529 HeLa cell RBPs, discovering numerous RNA-binding domains (RBDs). Catalytic centers or protein-protein interaction domains are in close relationship with RNA-binding sites, invoking possible effector roles of RNA in the control of protein function. Nearly half of the RNA-binding sites map to intrinsically disordered regions, uncovering unstructured domains as prevalent partners in protein-RNA interactions. RNA-binding sites represent hot spots for defined posttranslational modifications such as lysine acetylation and tyrosine phosphorylation, suggesting metabolic and signal-dependent regulation of RBP function. RBDs display a high degree of evolutionary conservation and incidence of Mendelian mutations, suggestive of important functional roles. RBDmap thus yields profound insights into native protein-RNA interactions in living cells. |
| spellingShingle | Castello, A Fischer, B Frese, C Horos, R Alleaume, A Foehr, S Curk, T Krijgsveld, J Hentze, M Comprehensive identification of RNA-binding domains in human cells |
| title | Comprehensive identification of RNA-binding domains in human cells |
| title_full | Comprehensive identification of RNA-binding domains in human cells |
| title_fullStr | Comprehensive identification of RNA-binding domains in human cells |
| title_full_unstemmed | Comprehensive identification of RNA-binding domains in human cells |
| title_short | Comprehensive identification of RNA-binding domains in human cells |
| title_sort | comprehensive identification of rna binding domains in human cells |
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