Dissociation of intact Escherichia coli ribosomes in a mass spectrometer. Evidence for conformational change in a ribosome elongation factor G complex.

We used mass spectrometry to identify proteins that are released in the gas phase from Escherichia coli ribosomes in response to a range of different solution conditions and cofactor binding. From solution at neutral pH the spectra are dominated by just 4 of the 54 ribosomal proteins (L7/L12, L11, a...

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Hlavní autoři: Hanson, C, Fucini, P, Ilag, L, Nierhaus, K, Robinson, C
Médium: Journal article
Jazyk:English
Vydáno: 2003