NMDA spikes enhance action potential generation during sensory input
Recent evidence in vitro suggests that the tuft dendrites of pyramidal neurons are capable of evoking local NMDA receptor-dependent electrogenesis, so-called NMDA spikes. However, it has so far proved difficult to demonstrate their existence in vivo. Moreover, it is not clear whether NMDA spikes are...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2014
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| _version_ | 1826284031434555392 |
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| author | Palmer, L Shai, A Reeve, J Anderson, H Paulsen, O Larkum, M |
| author_facet | Palmer, L Shai, A Reeve, J Anderson, H Paulsen, O Larkum, M |
| author_sort | Palmer, L |
| collection | OXFORD |
| description | Recent evidence in vitro suggests that the tuft dendrites of pyramidal neurons are capable of evoking local NMDA receptor-dependent electrogenesis, so-called NMDA spikes. However, it has so far proved difficult to demonstrate their existence in vivo. Moreover, it is not clear whether NMDA spikes are relevant to the output of pyramidal neurons. We found that local NMDA spikes occurred in tuft dendrites of layer 2/3 pyramidal neurons both spontaneously and following sensory input, and had a large influence on the number of output action potentials. Using two-photon activation of an intracellular caged NMDA receptor antagonist (tc-MK801), we found that isolated NMDA spikes typically occurred in multiple branches simultaneously and that sensory stimulation substantially increased their probability. Our results demonstrate that NMDA receptors have a vital role in coupling the tuft region of the layer 2/3 pyramidal neuron to the cell body, enhancing the effectiveness of layer 1 input. © 2014 Nature America, Inc. |
| first_indexed | 2024-03-07T01:07:44Z |
| format | Journal article |
| id | oxford-uuid:8bed65a3-c6fc-4465-8ff7-e47fe89ca31e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:07:44Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:8bed65a3-c6fc-4465-8ff7-e47fe89ca31e2022-03-26T22:41:19ZNMDA spikes enhance action potential generation during sensory inputJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8bed65a3-c6fc-4465-8ff7-e47fe89ca31eEnglishSymplectic Elements at Oxford2014Palmer, LShai, AReeve, JAnderson, HPaulsen, OLarkum, MRecent evidence in vitro suggests that the tuft dendrites of pyramidal neurons are capable of evoking local NMDA receptor-dependent electrogenesis, so-called NMDA spikes. However, it has so far proved difficult to demonstrate their existence in vivo. Moreover, it is not clear whether NMDA spikes are relevant to the output of pyramidal neurons. We found that local NMDA spikes occurred in tuft dendrites of layer 2/3 pyramidal neurons both spontaneously and following sensory input, and had a large influence on the number of output action potentials. Using two-photon activation of an intracellular caged NMDA receptor antagonist (tc-MK801), we found that isolated NMDA spikes typically occurred in multiple branches simultaneously and that sensory stimulation substantially increased their probability. Our results demonstrate that NMDA receptors have a vital role in coupling the tuft region of the layer 2/3 pyramidal neuron to the cell body, enhancing the effectiveness of layer 1 input. © 2014 Nature America, Inc. |
| spellingShingle | Palmer, L Shai, A Reeve, J Anderson, H Paulsen, O Larkum, M NMDA spikes enhance action potential generation during sensory input |
| title | NMDA spikes enhance action potential generation during sensory input |
| title_full | NMDA spikes enhance action potential generation during sensory input |
| title_fullStr | NMDA spikes enhance action potential generation during sensory input |
| title_full_unstemmed | NMDA spikes enhance action potential generation during sensory input |
| title_short | NMDA spikes enhance action potential generation during sensory input |
| title_sort | nmda spikes enhance action potential generation during sensory input |
| work_keys_str_mv | AT palmerl nmdaspikesenhanceactionpotentialgenerationduringsensoryinput AT shaia nmdaspikesenhanceactionpotentialgenerationduringsensoryinput AT reevej nmdaspikesenhanceactionpotentialgenerationduringsensoryinput AT andersonh nmdaspikesenhanceactionpotentialgenerationduringsensoryinput AT paulseno nmdaspikesenhanceactionpotentialgenerationduringsensoryinput AT larkumm nmdaspikesenhanceactionpotentialgenerationduringsensoryinput |