Enhanced protein adsorption upon bulk phase separation
In all areas related to protein adsorption, from medicine to biotechnology to heterogeneous nucleation, the question about its dominant forces and control arises. In this study, we used ellipsometry and quartz-crystal microbalance with dissipation (QCM-D), as well as density-functional theory (DFT)...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
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Nature Reaserch
2020
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| _version_ | 1826284046161805312 |
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| author | Fries, MR Stopper, D Skoda, MWA Blum, M Kertzscher, C Hinderhofer, A Zhang, F Jacobs, RMJ Roth, R Schreiber, F |
| author_facet | Fries, MR Stopper, D Skoda, MWA Blum, M Kertzscher, C Hinderhofer, A Zhang, F Jacobs, RMJ Roth, R Schreiber, F |
| author_sort | Fries, MR |
| collection | OXFORD |
| description | In all areas related to protein adsorption, from medicine to biotechnology to heterogeneous nucleation, the question about its dominant forces and control arises. In this study, we used ellipsometry and quartz-crystal microbalance with dissipation (QCM-D), as well as density-functional theory (DFT) to obtain insight into the mechanism behind a wetting transition of a protein solution. We established that using multivalent ions in a net negatively charged globular protein solution (BSA) can either cause simple adsorption on a negatively charged interface, or a (diverging) wetting layer when approaching liquid-liquid phase separation (LLPS) by changing protein concentration (cp) or temperature (T). We observed that the water to protein ratio in the wetting layer is substantially larger compared to simple adsorption. In the corresponding theoretical model, we treated the proteins as limited-valence (patchy) particles and identified a wetting transition for this complex system. This wetting is driven by a bulk instability introduced by metastable LLPS exposed to an ion-activated attractive substrate.
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| first_indexed | 2024-03-07T01:07:58Z |
| format | Journal article |
| id | oxford-uuid:8bff906b-e6ee-4ab0-932e-1e504d5cb0b3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:07:58Z |
| publishDate | 2020 |
| publisher | Nature Reaserch |
| record_format | dspace |
| spelling | oxford-uuid:8bff906b-e6ee-4ab0-932e-1e504d5cb0b32022-03-26T22:41:52ZEnhanced protein adsorption upon bulk phase separationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8bff906b-e6ee-4ab0-932e-1e504d5cb0b3EnglishSymplectic ElementsNature Reaserch2020Fries, MRStopper, DSkoda, MWABlum, MKertzscher, CHinderhofer, AZhang, FJacobs, RMJRoth, RSchreiber, FIn all areas related to protein adsorption, from medicine to biotechnology to heterogeneous nucleation, the question about its dominant forces and control arises. In this study, we used ellipsometry and quartz-crystal microbalance with dissipation (QCM-D), as well as density-functional theory (DFT) to obtain insight into the mechanism behind a wetting transition of a protein solution. We established that using multivalent ions in a net negatively charged globular protein solution (BSA) can either cause simple adsorption on a negatively charged interface, or a (diverging) wetting layer when approaching liquid-liquid phase separation (LLPS) by changing protein concentration (cp) or temperature (T). We observed that the water to protein ratio in the wetting layer is substantially larger compared to simple adsorption. In the corresponding theoretical model, we treated the proteins as limited-valence (patchy) particles and identified a wetting transition for this complex system. This wetting is driven by a bulk instability introduced by metastable LLPS exposed to an ion-activated attractive substrate. |
| spellingShingle | Fries, MR Stopper, D Skoda, MWA Blum, M Kertzscher, C Hinderhofer, A Zhang, F Jacobs, RMJ Roth, R Schreiber, F Enhanced protein adsorption upon bulk phase separation |
| title | Enhanced protein adsorption upon bulk phase separation |
| title_full | Enhanced protein adsorption upon bulk phase separation |
| title_fullStr | Enhanced protein adsorption upon bulk phase separation |
| title_full_unstemmed | Enhanced protein adsorption upon bulk phase separation |
| title_short | Enhanced protein adsorption upon bulk phase separation |
| title_sort | enhanced protein adsorption upon bulk phase separation |
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