Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles.
Marginal zone (MZ) B cells differ from follicular (FO) B cells in their functional, phenotypic and localization properties. It is still unclear whether B cells from the MZ compartment also have distinct or biased BCR specificities, recognizing only a limited number of conserved antigenic structures....
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2007
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| _version_ | 1797080984215093248 |
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| author | Gatto, D Bauer, M Martin, S Bachmann, M |
| author_facet | Gatto, D Bauer, M Martin, S Bachmann, M |
| author_sort | Gatto, D |
| collection | OXFORD |
| description | Marginal zone (MZ) B cells differ from follicular (FO) B cells in their functional, phenotypic and localization properties. It is still unclear whether B cells from the MZ compartment also have distinct or biased BCR specificities, recognizing only a limited number of conserved antigenic structures. To address the complexity of the immune response mounted by marginal zone B cells, we compared the antibody repertoire of murine MZ and FO B cells induced by immunization with two different virus-like particles (VLPs). Antibody sequences isolated from sorted VLP-specific MZ and FO B cells were similar in heavy chain V, D and J gene segment usage. Sequence analysis of CDR3 regions of antibodies from MZ and FO B cells also revealed no consistent difference in N nucleotide additions or CDR3 length. In contrast, somatic hypermutations were reduced in CDR regions of antibodies from MZ B cells compared to those from FO B cells. These results indicate that the response of MZ B cells to VLPs is clonotypically heterogeneous and suggest that the MZ B cell compartment is capable of generating variable and diverse antibody responses. |
| first_indexed | 2024-03-07T01:08:03Z |
| format | Journal article |
| id | oxford-uuid:8c06ee50-8fc9-4ece-9274-2af2c52d52c2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:08:03Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:8c06ee50-8fc9-4ece-9274-2af2c52d52c22022-03-26T22:41:56ZHeterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8c06ee50-8fc9-4ece-9274-2af2c52d52c2EnglishSymplectic Elements at Oxford2007Gatto, DBauer, MMartin, SBachmann, MMarginal zone (MZ) B cells differ from follicular (FO) B cells in their functional, phenotypic and localization properties. It is still unclear whether B cells from the MZ compartment also have distinct or biased BCR specificities, recognizing only a limited number of conserved antigenic structures. To address the complexity of the immune response mounted by marginal zone B cells, we compared the antibody repertoire of murine MZ and FO B cells induced by immunization with two different virus-like particles (VLPs). Antibody sequences isolated from sorted VLP-specific MZ and FO B cells were similar in heavy chain V, D and J gene segment usage. Sequence analysis of CDR3 regions of antibodies from MZ and FO B cells also revealed no consistent difference in N nucleotide additions or CDR3 length. In contrast, somatic hypermutations were reduced in CDR regions of antibodies from MZ B cells compared to those from FO B cells. These results indicate that the response of MZ B cells to VLPs is clonotypically heterogeneous and suggest that the MZ B cell compartment is capable of generating variable and diverse antibody responses. |
| spellingShingle | Gatto, D Bauer, M Martin, S Bachmann, M Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title | Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title_full | Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title_fullStr | Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title_full_unstemmed | Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title_short | Heterogeneous antibody repertoire of marginal zone B cells specific for virus-like particles. |
| title_sort | heterogeneous antibody repertoire of marginal zone b cells specific for virus like particles |
| work_keys_str_mv | AT gattod heterogeneousantibodyrepertoireofmarginalzonebcellsspecificforviruslikeparticles AT bauerm heterogeneousantibodyrepertoireofmarginalzonebcellsspecificforviruslikeparticles AT martins heterogeneousantibodyrepertoireofmarginalzonebcellsspecificforviruslikeparticles AT bachmannm heterogeneousantibodyrepertoireofmarginalzonebcellsspecificforviruslikeparticles |