Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside
<p>We have investigated the formation of supported model membranes via the adsorption of phospholipid–surfactant mixtures at the Si–water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant β-D-dodecyl maltoside and DOPC or POPC was determined...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2005
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| _version_ | 1826284087144349696 |
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| author | Vacklin, H Tiberg, F Thomas, R |
| author_facet | Vacklin, H Tiberg, F Thomas, R |
| author_sort | Vacklin, H |
| collection | OXFORD |
| description | <p>We have investigated the formation of supported model membranes via the adsorption of phospholipid–surfactant mixtures at the Si–water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant β-D-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d<sub>25</sub>-β-D-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39&pm;3 Å for DOPC and 41&pm;3 Å for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 Å<sup>2</sup> by varying the bulk concentrations used. The amount of surfactant in the bilayer is very sensitive to the bulk volume-to-surface area ratio, but it can be fully eliminated by ensuring a sufficiently large dilution/rinsing volume of the solution.</p> |
| first_indexed | 2024-03-07T01:08:35Z |
| format | Journal article |
| id | oxford-uuid:8c329837-c3f4-4d74-a8bb-60b7a82ba663 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:08:35Z |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:8c329837-c3f4-4d74-a8bb-60b7a82ba6632022-03-26T22:43:07ZFormation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltosideJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8c329837-c3f4-4d74-a8bb-60b7a82ba663Life SciencesEnglishOxford University Research Archive - ValetElsevier2005Vacklin, HTiberg, FThomas, R<p>We have investigated the formation of supported model membranes via the adsorption of phospholipid–surfactant mixtures at the Si–water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant β-D-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d<sub>25</sub>-β-D-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39&pm;3 Å for DOPC and 41&pm;3 Å for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 Å<sup>2</sup> by varying the bulk concentrations used. The amount of surfactant in the bilayer is very sensitive to the bulk volume-to-surface area ratio, but it can be fully eliminated by ensuring a sufficiently large dilution/rinsing volume of the solution.</p> |
| spellingShingle | Life Sciences Vacklin, H Tiberg, F Thomas, R Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title | Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title_full | Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title_fullStr | Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title_full_unstemmed | Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title_short | Formation of supported phospholipid bilayers via co-adsorption with β-D-dodecyl maltoside |
| title_sort | formation of supported phospholipid bilayers via co adsorption with β d dodecyl maltoside |
| topic | Life Sciences |
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