Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes.
The effect of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of HLA-DR, and the production of the cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha) by human peripheral blood monocyte-enriched populations was investigated. GM-CSF...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1990
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| _version_ | 1797081025201831936 |
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| author | Chantry, D Turner, M Brennan, F Kingsbury, A Feldmann, M |
| author_facet | Chantry, D Turner, M Brennan, F Kingsbury, A Feldmann, M |
| author_sort | Chantry, D |
| collection | OXFORD |
| description | The effect of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of HLA-DR, and the production of the cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha) by human peripheral blood monocyte-enriched populations was investigated. GM-CSF was shown to induce both the expression of HLA-DR and the cytokines IL-1 and TNF alpha in a dose-dependent manner. In contrast, interferon-gamma (IFN-gamma), which induced major histocompatibility complex (MHC) class II expression, did not induce IL-1 or TNF alpha production. However, IFN-gamma enhanced the cell surface expression of HLA-DR and the production of IL-1 and TNF alpha on monocyte-enriched cells stimulated by GM-CSF. By itself, GM-CSF did not induce surface class II expression on the human monocytic tumour cell line THP-1, whereas it synergized with IFN-gamma to induce surface expression. These cells responded to GM-CSF by producing IL-1 and TNF alpha; Northern blotting showed that mRNA levels of IL-1 and TNF alpha were transiently induced, similar to other cytokines. Our results indicate that GM-CSF is a major macrophage activating factor that is capable of inducing both the expression of HLA-DR and the cytokines involved in T-cell activation by macrophages; therefore, GM-CSF may be of importance in potentiating antigen presenting function. |
| first_indexed | 2024-03-07T01:08:40Z |
| format | Journal article |
| id | oxford-uuid:8c378b75-30dc-43ac-90cb-b06e21d49321 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:08:40Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:8c378b75-30dc-43ac-90cb-b06e21d493212022-03-26T22:43:19ZGranulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8c378b75-30dc-43ac-90cb-b06e21d49321EnglishSymplectic Elements at Oxford1990Chantry, DTurner, MBrennan, FKingsbury, AFeldmann, MThe effect of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of HLA-DR, and the production of the cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha) by human peripheral blood monocyte-enriched populations was investigated. GM-CSF was shown to induce both the expression of HLA-DR and the cytokines IL-1 and TNF alpha in a dose-dependent manner. In contrast, interferon-gamma (IFN-gamma), which induced major histocompatibility complex (MHC) class II expression, did not induce IL-1 or TNF alpha production. However, IFN-gamma enhanced the cell surface expression of HLA-DR and the production of IL-1 and TNF alpha on monocyte-enriched cells stimulated by GM-CSF. By itself, GM-CSF did not induce surface class II expression on the human monocytic tumour cell line THP-1, whereas it synergized with IFN-gamma to induce surface expression. These cells responded to GM-CSF by producing IL-1 and TNF alpha; Northern blotting showed that mRNA levels of IL-1 and TNF alpha were transiently induced, similar to other cytokines. Our results indicate that GM-CSF is a major macrophage activating factor that is capable of inducing both the expression of HLA-DR and the cytokines involved in T-cell activation by macrophages; therefore, GM-CSF may be of importance in potentiating antigen presenting function. |
| spellingShingle | Chantry, D Turner, M Brennan, F Kingsbury, A Feldmann, M Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title | Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title_full | Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title_fullStr | Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title_full_unstemmed | Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title_short | Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. |
| title_sort | granulocyte macrophage colony stimulating factor induces both hla dr expression and cytokine production by human monocytes |
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