Nano-engineering of materials for nonlinear optical imaging
We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical respo...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826284121958121472 |
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| author | Clays, K Vanderleyden, J Anderson, H |
| author_facet | Clays, K Vanderleyden, J Anderson, H |
| author_sort | Clays, K |
| collection | OXFORD |
| description | We discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system. © 2009 SPIE. |
| first_indexed | 2024-03-07T01:09:07Z |
| format | Journal article |
| id | oxford-uuid:8c60caa4-37ab-423c-91de-d8e4c9e9d7a3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:09:07Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:8c60caa4-37ab-423c-91de-d8e4c9e9d7a32022-03-26T22:44:10ZNano-engineering of materials for nonlinear optical imagingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8c60caa4-37ab-423c-91de-d8e4c9e9d7a3EnglishSymplectic Elements at Oxford2009Clays, KVanderleyden, JAnderson, HWe discuss the use of genetically modified fluorescent proteins (FPs) and a specially engineered chromophore for nonlinear optical imaging. While it is clear that FPs can be used for two-photon fluorescence (TPF) microscopy, we show that they also exhibit a large second-order nonlinear optical response, useful for second-harmonic generation (SHG) imaging. The relation between the linear and nonlinear optical properties in a small series of FPs will be discussed. We also present a new optimized chromophore for combined TPF and SHG microscopy and we show imaging results obtained on this chromophore on a water droplet model system. © 2009 SPIE. |
| spellingShingle | Clays, K Vanderleyden, J Anderson, H Nano-engineering of materials for nonlinear optical imaging |
| title | Nano-engineering of materials for nonlinear optical imaging |
| title_full | Nano-engineering of materials for nonlinear optical imaging |
| title_fullStr | Nano-engineering of materials for nonlinear optical imaging |
| title_full_unstemmed | Nano-engineering of materials for nonlinear optical imaging |
| title_short | Nano-engineering of materials for nonlinear optical imaging |
| title_sort | nano engineering of materials for nonlinear optical imaging |
| work_keys_str_mv | AT claysk nanoengineeringofmaterialsfornonlinearopticalimaging AT vanderleydenj nanoengineeringofmaterialsfornonlinearopticalimaging AT andersonh nanoengineeringofmaterialsfornonlinearopticalimaging |