A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor.

A modular and optimized single marker system for generating Trypanosoma brucei cell lines expressing T7 RNA polymerase and the tetracycline repressor.

Here, we present a simple modular extendable vector system for introducing the T7 RNA polymerase and tetracycline repressor genes into Trypanosoma brucei. This novel system exploits developments in our understanding of gene expression and genome organization to produce a streamlined plasmid optimize...

Descripció completa

Dades bibliogràfiques
Autors principals:	Poon, S, Peacock, L, Gibson, W, Gull, K, Kelly, S
Format:	Journal article
Idioma:	English
Publicat:	Royal Society 2012

Ítems similars

A new generation of T7 RNA polymerase-independent inducible expression plasmids for Trypanosoma brucei
per: Sunter, J, et al.
Publicat: (2012)

Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei.
per: Wickstead, B, et al.
Publicat: (2002)

The cell division cycle of Trypanosoma brucei brucei: timing of event markers and cytoskeletal modulations.
per: Sherwin, T, et al.
Publicat: (1989)

THE CELL-DIVISION CYCLE OF TRYPANOSOMA-BRUCEI-BRUCEI - TIMING OF EVENT MARKERS AND CYTOSKELETAL MODULATIONS
per: Sherwin, T, et al.
Publicat: (1989)

Signatures of hybridization in Trypanosoma brucei.
per: Christopher Kay, et al.
Publicat: (2022-02-01)

The heart of darkness: growth and form of Trypanosoma brucei in the tsetse fly.
per: Sharma, R, et al.
Publicat: (2009)

T-tubulin in Trypanosoma brucei
per: Scott, V, et al.
Publicat: (1996)

The heart of darkness : growth and form of Trypanosoma brucei in the tsetse fly.
per: Kumar Sharma, Reuben Sunil, et al.
Publicat: (2009)

Purification and assembly in vitro of tubulin from Trypanosoma brucei brucei.
per: MacRae, T, et al.
Publicat: (1990)

Mating compatibility in the parasitic protist Trypanosoma brucei
per: Lori Peacock, et al.
Publicat: (2014-02-01)

Double-stranded RNA induces mRNA degradation in Trypanosoma brucei.
per: Ngô, H, et al.
Publicat: (1998)

The bloodstream differentiation-division of Trypanosoma brucei studied using mitochondrial markers.
per: Tyler, K, et al.
Publicat: (1997)

Partitioning of large and minichromosomes in Trypanosoma brucei.
per: Ersfeld, K, et al.
Publicat: (1997)

Nuclear and genome organization of Trypanosoma brucei.
per: Ersfeld, K, et al.
Publicat: (1999)

The mitotic stability of the minichromosomes of Trypanosoma brucei.
per: Wickstead, B, et al.
Publicat: (2003)

The structural mechanics of cell division in Trypanosoma brucei.
per: Vaughan, S, et al.
Publicat: (2008)

Ultrastructural investigation methods for Trypanosoma brucei.
per: Höög, J, et al.
Publicat: (2010)

Targeting of cytoskeletal proteins to the flagellum of Trypanosoma brucei.
per: Ersfeld, K, et al.
Publicat: (2001)

Proteomics and the Trypanosoma brucei cytoskeleton: advances and opportunities.
per: Portman, N, et al.
Publicat: (2012)

Intraclonal mating occurs during tsetse transmission of <it>Trypanosoma brucei</it>
per: Ferris Vanessa, et al.
Publicat: (2009-09-01)

Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II in Trypanosoma brucei
per: Rudo Kieft, et al.
Publicat: (2025-02-01)

Diversity and dynamics of the minichromosomal karyotype in Trypanosoma brucei.
per: Alsford, S, et al.
Publicat: (2001)

The use of yellow fluorescent hybrids to indicate mating in <it>Trypanosoma brucei</it>
per: Ferris Vanessa, et al.
Publicat: (2008-02-01)

Subpellicular and flagellar microtubules of Trypanosoma brucei brucei contain the same alpha-tubulin isoforms.
per: Schneider, A, et al.
Publicat: (1987)

The configuration of DNA replication sites within the Trypanosoma brucei kinetoplast.
per: Robinson, DR, et al.
Publicat: (1994)

The cell cycle and cytoskeletal morphogenesis in Trypanosoma brucei.
per: Gull, K, et al.
Publicat: (1990)

Architecture of the Trypanosoma brucei nucleus during interphase and mitosis.
per: Ogbadoyi, E, et al.
Publicat: (2000)

Structural and Biochemical Characterization of Poly-ADP-ribose Polymerase from Trypanosoma brucei
per: Teemu Haikarainen, et al.
Publicat: (2017-06-01)

Manipulation of mitochondrial poly(A) polymerase family proteins in Trypanosoma brucei impacts mRNA termini processing
per: Clara M. Smoniewski, et al.
Publicat: (2024-01-01)

Tubulin post-translational modifications and the construction of microtubular organelles in Trypanosoma brucei.
per: Sasse, R, et al.
Publicat: (1988)

Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly
per: Peacock, L, et al.
Publicat: (2011)

SUMOylation in Trypanosoma brucei
per: Cornelia Andrea Klein, et al.
Publicat: (2013-10-01)

Modes of flagellar assembly in Chlamydomonas reinhardtii and Trypanosoma brucei
per: Höög, J, et al.
Publicat: (2014)

Depletion of the RNA-binding protein RBP33 results in increased expression of silenced RNA polymerase II transcripts in Trypanosoma brucei.
per: Sandra M Fernández-Moya, et al.
Publicat: (2014-01-01)

A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod.
per: Lacomble, S, et al.
Publicat: (2009)

Distinct localization and cell cycle dependence of COOH terminally tyrosinolated alpha-tubulin in the microtubules of Trypanosoma brucei brucei.
per: Sherwin, T, et al.
Publicat: (1987)

Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes
per: S Andrea Moreno, et al.
Publicat: (2015-06-01)

Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly.
per: Peacock, L., et al.
Publicat: (2011)

Early interactions between Trypanosoma brucei and the innate immune system
per: Griffiths, SR, et al.
Publicat: (2005)

Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast.
per: Gluenz, E, et al.
Publicat: (2007)