Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues.
The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1992
|
| _version_ | 1797081245768744960 |
|---|---|
| author | Steinkasserer, A Solari, R Mott, H Aplin, RT Robinson, C Willis, A Sim, R |
| author_facet | Steinkasserer, A Solari, R Mott, H Aplin, RT Robinson, C Willis, A Sim, R |
| author_sort | Steinkasserer, A |
| collection | OXFORD |
| description | The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked. |
| first_indexed | 2024-03-07T01:11:52Z |
| format | Journal article |
| id | oxford-uuid:8d4863cf-5d83-44ab-b4f0-a1fe70429c9f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:11:52Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:8d4863cf-5d83-44ab-b4f0-a1fe70429c9f2022-03-26T22:50:16ZHuman interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8d4863cf-5d83-44ab-b4f0-a1fe70429c9fEnglishSymplectic Elements at Oxford1992Steinkasserer, ASolari, RMott, HAplin, RTRobinson, CWillis, ASim, RThe human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked. |
| spellingShingle | Steinkasserer, A Solari, R Mott, H Aplin, RT Robinson, C Willis, A Sim, R Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title | Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title_full | Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title_fullStr | Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title_full_unstemmed | Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title_short | Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
| title_sort | human interleukin 1 receptor antagonist high yield expression in e coli and examination of cysteine residues |
| work_keys_str_mv | AT steinkasserera humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT solarir humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT motth humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT aplinrt humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT robinsonc humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT willisa humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues AT simr humaninterleukin1receptorantagonisthighyieldexpressioninecoliandexaminationofcysteineresidues |