Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology
Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large c...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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Rockefeller University Press
2014
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| _version_ | 1797111934918590464 |
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| author | Hayes, P Varga, V Olego-Fernandez, S Sunter, J Ginger, ML Gull, K |
| author_facet | Hayes, P Varga, V Olego-Fernandez, S Sunter, J Ginger, ML Gull, K |
| author_sort | Hayes, P |
| collection | OXFORD |
| description | Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components. |
| first_indexed | 2024-03-07T08:17:21Z |
| format | Journal article |
| id | oxford-uuid:8d819346-024d-43bd-9700-1d0dc945f598 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:17:21Z |
| publishDate | 2014 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:8d819346-024d-43bd-9700-1d0dc945f5982024-01-08T10:36:46ZModulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphologyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8d819346-024d-43bd-9700-1d0dc945f598EnglishSymplectic Elements at OxfordRockefeller University Press2014Hayes, PVarga, VOlego-Fernandez, SSunter, JGinger, MLGull, KIndividual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components. |
| spellingShingle | Hayes, P Varga, V Olego-Fernandez, S Sunter, J Ginger, ML Gull, K Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title | Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title_full | Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title_fullStr | Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title_full_unstemmed | Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title_short | Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology |
| title_sort | modulation of a cytoskeletal calpain like protein induces major transitions in trypanosome morphology |
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