Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex.
We have used systematic fluorescence resonance energy transfer and distance-constrained docking to define the three-dimensional structures of bacterial RNA polymerase holoenzyme and the bacterial RNA polymerase-promoter open complex in solution. The structures provide a framework for understanding s...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2002
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| _version_ | 1826284371333611520 |
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| author | Mekler, V Kortkhonjia, E Mukhopadhyay, J Knight, J Revyakin, A Kapanidis, A Niu, W Ebright, Y Levy, R Ebright, R |
| author_facet | Mekler, V Kortkhonjia, E Mukhopadhyay, J Knight, J Revyakin, A Kapanidis, A Niu, W Ebright, Y Levy, R Ebright, R |
| author_sort | Mekler, V |
| collection | OXFORD |
| description | We have used systematic fluorescence resonance energy transfer and distance-constrained docking to define the three-dimensional structures of bacterial RNA polymerase holoenzyme and the bacterial RNA polymerase-promoter open complex in solution. The structures provide a framework for understanding sigma(70)-(RNA polymerase core), sigma(70)-DNA, and sigma(70)-RNA interactions. The positions of sigma(70) regions 1.2, 2, 3, and 4 are similar in holoenzyme and open complex. In contrast, the position of sigma(70) region 1.1 differs dramatically in holoenzyme and open complex. In holoenzyme, region 1.1 is located within the active-center cleft, apparently serving as a "molecular mimic" of DNA, but, in open complex, region 1.1 is located outside the active center cleft. The approach described here should be applicable to the analysis of other nanometer-scale complexes. |
| first_indexed | 2024-03-07T01:12:50Z |
| format | Journal article |
| id | oxford-uuid:8d9f76c9-6d22-49f4-94a8-ef105095b048 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:12:50Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:8d9f76c9-6d22-49f4-94a8-ef105095b0482022-03-26T22:52:22ZStructural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8d9f76c9-6d22-49f4-94a8-ef105095b048EnglishSymplectic Elements at Oxford2002Mekler, VKortkhonjia, EMukhopadhyay, JKnight, JRevyakin, AKapanidis, ANiu, WEbright, YLevy, REbright, RWe have used systematic fluorescence resonance energy transfer and distance-constrained docking to define the three-dimensional structures of bacterial RNA polymerase holoenzyme and the bacterial RNA polymerase-promoter open complex in solution. The structures provide a framework for understanding sigma(70)-(RNA polymerase core), sigma(70)-DNA, and sigma(70)-RNA interactions. The positions of sigma(70) regions 1.2, 2, 3, and 4 are similar in holoenzyme and open complex. In contrast, the position of sigma(70) region 1.1 differs dramatically in holoenzyme and open complex. In holoenzyme, region 1.1 is located within the active-center cleft, apparently serving as a "molecular mimic" of DNA, but, in open complex, region 1.1 is located outside the active center cleft. The approach described here should be applicable to the analysis of other nanometer-scale complexes. |
| spellingShingle | Mekler, V Kortkhonjia, E Mukhopadhyay, J Knight, J Revyakin, A Kapanidis, A Niu, W Ebright, Y Levy, R Ebright, R Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title | Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title_full | Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title_fullStr | Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title_full_unstemmed | Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title_short | Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex. |
| title_sort | structural organization of bacterial rna polymerase holoenzyme and the rna polymerase promoter open complex |
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