Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B.
A molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recogn...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1797081329836228608 |
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| author | Li, Y Tee, K Liao, H Hase, S Uenishi, R Li, X Tsuchiura, T Yang, R Govindasamy, S Yong, Y Tan, H Pybus, O Kamarulzaman, A Takebe, Y |
| author_facet | Li, Y Tee, K Liao, H Hase, S Uenishi, R Li, X Tsuchiura, T Yang, R Govindasamy, S Yong, Y Tan, H Pybus, O Kamarulzaman, A Takebe, Y |
| author_sort | Li, Y |
| collection | OXFORD |
| description | A molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recognizable linkage revealed that they have an identical subtype structure comprised of CRF01_AE and subtype B', distinct from any known circulating recombinant forms (CRFs). This novel CRF, designated CRF48_01B, is closely related to CRF33_01B, previously identified in Kuala Lumpur. Phylogenetic analysis of multiple CRF48_01B genome regions showed that CRF48_01B forms a monophyletic cluster within CRF33_01B, suggesting that this new recombinant is very likely a descendant of CRF33_01B. CRF48_01B thus represents one of the first examples of a "second-generation" CRF, generated by additional crossover with pre-existing CRFs. Corroborating these results, Bayesian molecular clock analyses indicated that CRF48_01B emerged in approximately 2001, approximately approximately 8 years after the emergence of CRF33_01B. |
| first_indexed | 2024-03-07T01:13:01Z |
| format | Journal article |
| id | oxford-uuid:8db101cd-c7f0-432b-b198-bb5b0abe9013 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:13:01Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:8db101cd-c7f0-432b-b198-bb5b0abe90132022-03-26T22:52:48ZIdentification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8db101cd-c7f0-432b-b198-bb5b0abe9013EnglishSymplectic Elements at Oxford2010Li, YTee, KLiao, HHase, SUenishi, RLi, XTsuchiura, TYang, RGovindasamy, SYong, YTan, HPybus, OKamarulzaman, ATakebe, YA molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recognizable linkage revealed that they have an identical subtype structure comprised of CRF01_AE and subtype B', distinct from any known circulating recombinant forms (CRFs). This novel CRF, designated CRF48_01B, is closely related to CRF33_01B, previously identified in Kuala Lumpur. Phylogenetic analysis of multiple CRF48_01B genome regions showed that CRF48_01B forms a monophyletic cluster within CRF33_01B, suggesting that this new recombinant is very likely a descendant of CRF33_01B. CRF48_01B thus represents one of the first examples of a "second-generation" CRF, generated by additional crossover with pre-existing CRFs. Corroborating these results, Bayesian molecular clock analyses indicated that CRF48_01B emerged in approximately 2001, approximately approximately 8 years after the emergence of CRF33_01B. |
| spellingShingle | Li, Y Tee, K Liao, H Hase, S Uenishi, R Li, X Tsuchiura, T Yang, R Govindasamy, S Yong, Y Tan, H Pybus, O Kamarulzaman, A Takebe, Y Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title | Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title_full | Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title_fullStr | Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title_full_unstemmed | Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title_short | Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B. |
| title_sort | identification of a novel second generation circulating recombinant form crf48 01b in malaysia a descendant of the previously identified crf33 01b |
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