Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
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Springer Nature
2017
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| _version_ | 1826284399736389632 |
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| author | Folliard, T Mertins, B Steel, H Prescott, T Newport, T Jones, C Wadhams, G Bayer, T Armitage, J Papachristodoulou, A Rothschild, L |
| author_facet | Folliard, T Mertins, B Steel, H Prescott, T Newport, T Jones, C Wadhams, G Bayer, T Armitage, J Papachristodoulou, A Rothschild, L |
| author_sort | Folliard, T |
| collection | OXFORD |
| description | Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications. |
| first_indexed | 2024-03-07T01:13:16Z |
| format | Journal article |
| id | oxford-uuid:8dc525be-f1e4-4633-99c1-1898bc41ebc3 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:13:16Z |
| publishDate | 2017 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:8dc525be-f1e4-4633-99c1-1898bc41ebc32022-03-26T22:53:21ZRibo-attenuators: novel elements for reliable and modular riboswitch engineeringJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8dc525be-f1e4-4633-99c1-1898bc41ebc3Symplectic Elements at OxfordSpringer Nature2017Folliard, TMertins, BSteel, HPrescott, TNewport, TJones, CWadhams, GBayer, TArmitage, JPapachristodoulou, ARothschild, LRiboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications. |
| spellingShingle | Folliard, T Mertins, B Steel, H Prescott, T Newport, T Jones, C Wadhams, G Bayer, T Armitage, J Papachristodoulou, A Rothschild, L Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title | Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title_full | Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title_fullStr | Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title_full_unstemmed | Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title_short | Ribo-attenuators: novel elements for reliable and modular riboswitch engineering |
| title_sort | ribo attenuators novel elements for reliable and modular riboswitch engineering |
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