Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells.
1. This study investigates the acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. 2. After incubation of intact cells with 0.1 mmol/l 5-aminosalicylic acid, N-acetyl-5-aminosalicylic acid was detected in a concentration of 141 (+/- 23.8 SEM) nmol/g dry weight in the inc...
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| Format: | Journal article |
| Language: | English |
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1990
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| _version_ | 1826284445194256384 |
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| author | Ireland, A Priddle, J Jewell, D |
| author_facet | Ireland, A Priddle, J Jewell, D |
| author_sort | Ireland, A |
| collection | OXFORD |
| description | 1. This study investigates the acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. 2. After incubation of intact cells with 0.1 mmol/l 5-aminosalicylic acid, N-acetyl-5-aminosalicylic acid was detected in a concentration of 141 (+/- 23.8 SEM) nmol/g dry weight in the incubation medium, and 34.8 (+/- 5.5 SEM) nmol/g dry weight intracellularly. No unchanged 5-aminosalicylic acid was detected inside the cell. 3. Acetylation of 5-aminosalicylic acid by a cell homogenate was very poor, but the addition of 1 mmol/l acetyl-CoA resulted in complete conversion of 0.1 mmol/l 5-aminosalicylic acid to N-acetyl-5-aminosalicylic acid. 4. N-Acetyltransferase activity was detected in the cytosol, with a mean of 3.3 nmol min-1 mg-1 of protein. There was no N-acetyltransferase activity in the brush border. There was no difference in enzyme activity between epithelial cells derived from normal, Crohn's disease or ulcerative colitis patients. 5. Preliminary characterization of the N-acetyltransferase enzyme suggests a molecular weight of 150,000. |
| first_indexed | 2024-03-07T01:13:56Z |
| format | Journal article |
| id | oxford-uuid:8dff0385-be82-4470-88b4-08c0ace69d82 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:13:56Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:8dff0385-be82-4470-88b4-08c0ace69d822022-03-26T22:54:42ZAcetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8dff0385-be82-4470-88b4-08c0ace69d82EnglishSymplectic Elements at Oxford1990Ireland, APriddle, JJewell, D1. This study investigates the acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. 2. After incubation of intact cells with 0.1 mmol/l 5-aminosalicylic acid, N-acetyl-5-aminosalicylic acid was detected in a concentration of 141 (+/- 23.8 SEM) nmol/g dry weight in the incubation medium, and 34.8 (+/- 5.5 SEM) nmol/g dry weight intracellularly. No unchanged 5-aminosalicylic acid was detected inside the cell. 3. Acetylation of 5-aminosalicylic acid by a cell homogenate was very poor, but the addition of 1 mmol/l acetyl-CoA resulted in complete conversion of 0.1 mmol/l 5-aminosalicylic acid to N-acetyl-5-aminosalicylic acid. 4. N-Acetyltransferase activity was detected in the cytosol, with a mean of 3.3 nmol min-1 mg-1 of protein. There was no N-acetyltransferase activity in the brush border. There was no difference in enzyme activity between epithelial cells derived from normal, Crohn's disease or ulcerative colitis patients. 5. Preliminary characterization of the N-acetyltransferase enzyme suggests a molecular weight of 150,000. |
| spellingShingle | Ireland, A Priddle, J Jewell, D Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title | Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title_full | Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title_fullStr | Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title_full_unstemmed | Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title_short | Acetylation of 5-aminosalicylic acid by isolated human colonic epithelial cells. |
| title_sort | acetylation of 5 aminosalicylic acid by isolated human colonic epithelial cells |
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