Proteomic analysis of Leishmania mexicana differentiation.
We have resolved the proteome of axenically differentiated Leishmania mexicana parasites by two-dimensional gel electrophoresis (2DE), employing optimised, robust and reproducible procedures, and visualised (by silver staining) approximately 2000 protein species in each of three developmental stages...
| Egile Nagusiak: | , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
2004
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| _version_ | 1826284447050235904 |
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| author | Nugent, P Karsani, SA Wait, R Tempero, J Smith, D |
| author_facet | Nugent, P Karsani, SA Wait, R Tempero, J Smith, D |
| author_sort | Nugent, P |
| collection | OXFORD |
| description | We have resolved the proteome of axenically differentiated Leishmania mexicana parasites by two-dimensional gel electrophoresis (2DE), employing optimised, robust and reproducible procedures, and visualised (by silver staining) approximately 2000 protein species in each of three developmental stages: procyclic promastigotes, metacyclic promastigotes and amastigotes. This analysis has used homogeneous populations of these parasite stages, characterised according to their morphology, protease and nuclease activity profiles and expression of stage-specific antigens. Following comparison of the whole proteome profiles between stages, 47 spots were found to be stage-specific, while a further 100 spots changed in intensity during differentiation. The majority of "unique" spots were expressed during the infective stages of parasite differentiation, metacyclic promastigotes and amastigotes. CapLC-QTOF mass spectrometry has allowed the identification of 47 protein species to date, including a number which are only detected in the amastigote stage. Proteins identified are members of eight functionally related groupings, some of which are implicated in infectivity and host-parasite interactions. |
| first_indexed | 2024-03-07T01:13:58Z |
| format | Journal article |
| id | oxford-uuid:8e01589b-c8ed-4ba3-baf6-8898c96ab1a4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:13:58Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:8e01589b-c8ed-4ba3-baf6-8898c96ab1a42022-03-26T22:54:48ZProteomic analysis of Leishmania mexicana differentiation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8e01589b-c8ed-4ba3-baf6-8898c96ab1a4EnglishSymplectic Elements at Oxford2004Nugent, PKarsani, SAWait, RTempero, JSmith, DWe have resolved the proteome of axenically differentiated Leishmania mexicana parasites by two-dimensional gel electrophoresis (2DE), employing optimised, robust and reproducible procedures, and visualised (by silver staining) approximately 2000 protein species in each of three developmental stages: procyclic promastigotes, metacyclic promastigotes and amastigotes. This analysis has used homogeneous populations of these parasite stages, characterised according to their morphology, protease and nuclease activity profiles and expression of stage-specific antigens. Following comparison of the whole proteome profiles between stages, 47 spots were found to be stage-specific, while a further 100 spots changed in intensity during differentiation. The majority of "unique" spots were expressed during the infective stages of parasite differentiation, metacyclic promastigotes and amastigotes. CapLC-QTOF mass spectrometry has allowed the identification of 47 protein species to date, including a number which are only detected in the amastigote stage. Proteins identified are members of eight functionally related groupings, some of which are implicated in infectivity and host-parasite interactions. |
| spellingShingle | Nugent, P Karsani, SA Wait, R Tempero, J Smith, D Proteomic analysis of Leishmania mexicana differentiation. |
| title | Proteomic analysis of Leishmania mexicana differentiation. |
| title_full | Proteomic analysis of Leishmania mexicana differentiation. |
| title_fullStr | Proteomic analysis of Leishmania mexicana differentiation. |
| title_full_unstemmed | Proteomic analysis of Leishmania mexicana differentiation. |
| title_short | Proteomic analysis of Leishmania mexicana differentiation. |
| title_sort | proteomic analysis of leishmania mexicana differentiation |
| work_keys_str_mv | AT nugentp proteomicanalysisofleishmaniamexicanadifferentiation AT karsanisa proteomicanalysisofleishmaniamexicanadifferentiation AT waitr proteomicanalysisofleishmaniamexicanadifferentiation AT temperoj proteomicanalysisofleishmaniamexicanadifferentiation AT smithd proteomicanalysisofleishmaniamexicanadifferentiation |