Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B.
Increasing attention has been focused on the use of recombinant mammalian viruses as potential vaccines. Recombinant human adenoviruses are one of the more promising vaccine vectors because they can be easily constructed and because live adenovirus vaccines have been administered orally to large num...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1989
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| _version_ | 1797081590621274112 |
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| author | McDermott, MR Graham, F Hanke, T Johnson, D |
| author_facet | McDermott, MR Graham, F Hanke, T Johnson, D |
| author_sort | McDermott, MR |
| collection | OXFORD |
| description | Increasing attention has been focused on the use of recombinant mammalian viruses as potential vaccines. Recombinant human adenoviruses are one of the more promising vaccine vectors because they can be easily constructed and because live adenovirus vaccines have been administered orally to large numbers of military recruits without adverse reactions. In order to examine the efficacy of human adenoviruses as vaccines we have studied the immunity induced by a recombinant adenovirus vector, AdgB2, which induces high level expression of herpes simplex virus (HSV) glycoprotein B (gB) in human and murine cells. Mice inoculated with AdgB2 produced antibodies specific for gB which neutralized HSV in the presence of complement. Although mice inoculated with AdgB2 showed no ill-effects after AdgB2 inoculation and we were unable to detect replication of human adenoviruses in mice, the mice were protected from a lethal challenge with HSV after a single inoculation with AdgB2. |
| first_indexed | 2024-03-07T01:16:21Z |
| format | Journal article |
| id | oxford-uuid:8ed32b86-3c41-4548-8d0e-fadc51f69f91 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:16:21Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:8ed32b86-3c41-4548-8d0e-fadc51f69f912022-03-26T23:00:11ZProtection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8ed32b86-3c41-4548-8d0e-fadc51f69f91EnglishSymplectic Elements at Oxford1989McDermott, MRGraham, FHanke, TJohnson, DIncreasing attention has been focused on the use of recombinant mammalian viruses as potential vaccines. Recombinant human adenoviruses are one of the more promising vaccine vectors because they can be easily constructed and because live adenovirus vaccines have been administered orally to large numbers of military recruits without adverse reactions. In order to examine the efficacy of human adenoviruses as vaccines we have studied the immunity induced by a recombinant adenovirus vector, AdgB2, which induces high level expression of herpes simplex virus (HSV) glycoprotein B (gB) in human and murine cells. Mice inoculated with AdgB2 produced antibodies specific for gB which neutralized HSV in the presence of complement. Although mice inoculated with AdgB2 showed no ill-effects after AdgB2 inoculation and we were unable to detect replication of human adenoviruses in mice, the mice were protected from a lethal challenge with HSV after a single inoculation with AdgB2. |
| spellingShingle | McDermott, MR Graham, F Hanke, T Johnson, D Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title | Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title_full | Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title_fullStr | Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title_full_unstemmed | Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title_short | Protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing HSV glycoprotein B. |
| title_sort | protection of mice against lethal challenge with herpes simplex virus by vaccination with an adenovirus vector expressing hsv glycoprotein b |
| work_keys_str_mv | AT mcdermottmr protectionofmiceagainstlethalchallengewithherpessimplexvirusbyvaccinationwithanadenovirusvectorexpressinghsvglycoproteinb AT grahamf protectionofmiceagainstlethalchallengewithherpessimplexvirusbyvaccinationwithanadenovirusvectorexpressinghsvglycoproteinb AT hanket protectionofmiceagainstlethalchallengewithherpessimplexvirusbyvaccinationwithanadenovirusvectorexpressinghsvglycoproteinb AT johnsond protectionofmiceagainstlethalchallengewithherpessimplexvirusbyvaccinationwithanadenovirusvectorexpressinghsvglycoproteinb |