RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line
<br><strong>Background: </strong>The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement. <br><strong>Methods: </strong>We p...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
F1000 Research
2020
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| _version_ | 1797081613916438528 |
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| author | Felce, SL Farnie, G Dustin, ML Felce, JH |
| author_facet | Felce, SL Farnie, G Dustin, ML Felce, JH |
| author_sort | Felce, SL |
| collection | OXFORD |
| description | <br><strong>Background: </strong>The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement.
<br><strong>Methods: </strong>We present whole-transcriptome RNA-Sequencing data for Jurkat E6.1 cells in the resting state and two hours post-activation via TCR and CD28. We compare early transcriptional responses in the presence and absence of the chemokines CXCL12 and CCL19, and perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells using publicly deposited data.
<br><strong>Results: </strong>Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the depth of responses in primary cells.
<br><strong>Conclusions: </strong>These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional responses.
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| first_indexed | 2024-03-07T01:16:41Z |
| format | Journal article |
| id | oxford-uuid:8eeea15f-18d0-4a04-880e-dd2a3acecf40 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:16:41Z |
| publishDate | 2020 |
| publisher | F1000 Research |
| record_format | dspace |
| spelling | oxford-uuid:8eeea15f-18d0-4a04-880e-dd2a3acecf402022-03-26T23:01:00ZRNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell lineJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8eeea15f-18d0-4a04-880e-dd2a3acecf40EnglishSymplectic ElementsF1000 Research2020Felce, SLFarnie, GDustin, MLFelce, JH<br><strong>Background: </strong>The leukaemia-derived Jurkat E6.1 cell line has been used as a model T cell in the study of many aspects of T cell biology, most notably activation in response to T cell receptor (TCR) engagement. <br><strong>Methods: </strong>We present whole-transcriptome RNA-Sequencing data for Jurkat E6.1 cells in the resting state and two hours post-activation via TCR and CD28. We compare early transcriptional responses in the presence and absence of the chemokines CXCL12 and CCL19, and perform a basic comparison between observed transcriptional responses in Jurkat E6.1 cells and those in primary human T cells using publicly deposited data. <br><strong>Results: </strong>Jurkat E6.1 cells have many of the hallmarks of standard T cell transcriptional responses to activation, but lack most of the depth of responses in primary cells. <br><strong>Conclusions: </strong>These data indicate that Jurkat E6.1 cells hence represent only a highly simplified model of early T cell transcriptional responses. |
| spellingShingle | Felce, SL Farnie, G Dustin, ML Felce, JH RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title | RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title_full | RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title_fullStr | RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title_full_unstemmed | RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title_short | RNA-Seq analysis of early transcriptional responses to activation in the leukaemic Jurkat E6.1 T cell line |
| title_sort | rna seq analysis of early transcriptional responses to activation in the leukaemic jurkat e6 1 t cell line |
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