In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
Purpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All cultur...
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Format: | Journal article |
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Nature Publishing Group
2011
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author | Orlans, H Hornby, S Bowler, I |
author_facet | Orlans, H Hornby, S Bowler, I |
author_sort | Orlans, H |
collection | OXFORD |
description | Purpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All culture-positive corneal scrapes received from the Oxford Eye Hospital between July 1999 and June 2009 were identified retrospectively using a local microbiology database. For analysis of trends over time, the data was split into two equal 5-year periods. Statistical analysis was done using the χ 2 and Fisher exact tests. Results Over the 10-year study period, 467 corneal scrapes were performed of which 252 (54.0%) had positive bacterial cultures, growing a total of 267 organisms. The most commonly isolated bacteria were Staphylococci(40.1%) followed by Pseudomonasspecies (28.5%), other Gram-negative species (17.2%), Streptococci(7.1%), and Corynebacteria(6.0%). Between the first and second time periods there was an increase in the number of coagulase-negative Staphylococciand an increased resistance of the non-Pseudomonas Gram-negative group to chloramphenicol. Of the 189 isolates tested for sensitivity to both empirical antibiotic regimens, 176 (93.2%) were susceptible to ciprofloxacin whereas 188 (99.5%) were susceptible to either gentamicin or cefuroxime (P=0.0015). Conclusions The spectrum of bacterial keratitis isolates and their in vitroantibiotic sensitivity patterns have generally remained stable over time. The combination of gentamicin and cefuroxime provides a broader spectrum of antimicrobial cover than ciprofloxacin monotherapy in Oxford, although both regimens continue to be appropriate choices for the initial management of this condition. |
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format | Journal article |
id | oxford-uuid:8f208cbe-d37f-4c8b-8d8f-c65080db6144 |
institution | University of Oxford |
last_indexed | 2024-03-07T01:17:17Z |
publishDate | 2011 |
publisher | Nature Publishing Group |
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spelling | oxford-uuid:8f208cbe-d37f-4c8b-8d8f-c65080db61442022-03-26T23:02:14ZIn vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year reviewJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8f208cbe-d37f-4c8b-8d8f-c65080db6144Symplectic Elements at OxfordNature Publishing Group2011Orlans, HHornby, SBowler, IPurpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All culture-positive corneal scrapes received from the Oxford Eye Hospital between July 1999 and June 2009 were identified retrospectively using a local microbiology database. For analysis of trends over time, the data was split into two equal 5-year periods. Statistical analysis was done using the χ 2 and Fisher exact tests. Results Over the 10-year study period, 467 corneal scrapes were performed of which 252 (54.0%) had positive bacterial cultures, growing a total of 267 organisms. The most commonly isolated bacteria were Staphylococci(40.1%) followed by Pseudomonasspecies (28.5%), other Gram-negative species (17.2%), Streptococci(7.1%), and Corynebacteria(6.0%). Between the first and second time periods there was an increase in the number of coagulase-negative Staphylococciand an increased resistance of the non-Pseudomonas Gram-negative group to chloramphenicol. Of the 189 isolates tested for sensitivity to both empirical antibiotic regimens, 176 (93.2%) were susceptible to ciprofloxacin whereas 188 (99.5%) were susceptible to either gentamicin or cefuroxime (P=0.0015). Conclusions The spectrum of bacterial keratitis isolates and their in vitroantibiotic sensitivity patterns have generally remained stable over time. The combination of gentamicin and cefuroxime provides a broader spectrum of antimicrobial cover than ciprofloxacin monotherapy in Oxford, although both regimens continue to be appropriate choices for the initial management of this condition. |
spellingShingle | Orlans, H Hornby, S Bowler, I In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title | In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title_full | In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title_fullStr | In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title_full_unstemmed | In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title_short | In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review |
title_sort | in vitro antibiotic susceptibility patterns of bacterial keratitis isolates in oxford uk a 10 year review |
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