In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review

Purpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All cultur...

Full description

Bibliographic Details
Main Authors: Orlans, H, Hornby, S, Bowler, I
Format: Journal article
Published: Nature Publishing Group 2011
_version_ 1797081665225359360
author Orlans, H
Hornby, S
Bowler, I
author_facet Orlans, H
Hornby, S
Bowler, I
author_sort Orlans, H
collection OXFORD
description Purpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All culture-positive corneal scrapes received from the Oxford Eye Hospital between July 1999 and June 2009 were identified retrospectively using a local microbiology database. For analysis of trends over time, the data was split into two equal 5-year periods. Statistical analysis was done using the χ 2 and Fisher exact tests. Results Over the 10-year study period, 467 corneal scrapes were performed of which 252 (54.0%) had positive bacterial cultures, growing a total of 267 organisms. The most commonly isolated bacteria were Staphylococci(40.1%) followed by Pseudomonasspecies (28.5%), other Gram-negative species (17.2%), Streptococci(7.1%), and Corynebacteria(6.0%). Between the first and second time periods there was an increase in the number of coagulase-negative Staphylococciand an increased resistance of the non-Pseudomonas Gram-negative group to chloramphenicol. Of the 189 isolates tested for sensitivity to both empirical antibiotic regimens, 176 (93.2%) were susceptible to ciprofloxacin whereas 188 (99.5%) were susceptible to either gentamicin or cefuroxime (P=0.0015). Conclusions The spectrum of bacterial keratitis isolates and their in vitroantibiotic sensitivity patterns have generally remained stable over time. The combination of gentamicin and cefuroxime provides a broader spectrum of antimicrobial cover than ciprofloxacin monotherapy in Oxford, although both regimens continue to be appropriate choices for the initial management of this condition.
first_indexed 2024-03-07T01:17:17Z
format Journal article
id oxford-uuid:8f208cbe-d37f-4c8b-8d8f-c65080db6144
institution University of Oxford
last_indexed 2024-03-07T01:17:17Z
publishDate 2011
publisher Nature Publishing Group
record_format dspace
spelling oxford-uuid:8f208cbe-d37f-4c8b-8d8f-c65080db61442022-03-26T23:02:14ZIn vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year reviewJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8f208cbe-d37f-4c8b-8d8f-c65080db6144Symplectic Elements at OxfordNature Publishing Group2011Orlans, HHornby, SBowler, IPurpose To analyse the spectrum of bacterial keratitis isolates and their in vitroantibiotic susceptibilities over a 10-year period in Oxford, UK; and to compare the in vitroefficacy of ciprofloxacin with that of the combination of gentamicin and cefuroxime over the same period. Methods All culture-positive corneal scrapes received from the Oxford Eye Hospital between July 1999 and June 2009 were identified retrospectively using a local microbiology database. For analysis of trends over time, the data was split into two equal 5-year periods. Statistical analysis was done using the χ 2 and Fisher exact tests. Results Over the 10-year study period, 467 corneal scrapes were performed of which 252 (54.0%) had positive bacterial cultures, growing a total of 267 organisms. The most commonly isolated bacteria were Staphylococci(40.1%) followed by Pseudomonasspecies (28.5%), other Gram-negative species (17.2%), Streptococci(7.1%), and Corynebacteria(6.0%). Between the first and second time periods there was an increase in the number of coagulase-negative Staphylococciand an increased resistance of the non-Pseudomonas Gram-negative group to chloramphenicol. Of the 189 isolates tested for sensitivity to both empirical antibiotic regimens, 176 (93.2%) were susceptible to ciprofloxacin whereas 188 (99.5%) were susceptible to either gentamicin or cefuroxime (P=0.0015). Conclusions The spectrum of bacterial keratitis isolates and their in vitroantibiotic sensitivity patterns have generally remained stable over time. The combination of gentamicin and cefuroxime provides a broader spectrum of antimicrobial cover than ciprofloxacin monotherapy in Oxford, although both regimens continue to be appropriate choices for the initial management of this condition.
spellingShingle Orlans, H
Hornby, S
Bowler, I
In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title_full In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title_fullStr In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title_full_unstemmed In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title_short In vitro antibiotic susceptibility patterns of bacterial keratitis isolates in Oxford, UK: a 10-year review
title_sort in vitro antibiotic susceptibility patterns of bacterial keratitis isolates in oxford uk a 10 year review
work_keys_str_mv AT orlansh invitroantibioticsusceptibilitypatternsofbacterialkeratitisisolatesinoxforduka10yearreview
AT hornbys invitroantibioticsusceptibilitypatternsofbacterialkeratitisisolatesinoxforduka10yearreview
AT bowleri invitroantibioticsusceptibilitypatternsofbacterialkeratitisisolatesinoxforduka10yearreview