Projection structure of a transcriptional regulator, HupR, determined by electron cryo-microscopy.
Large, well-ordered two-dimensional crystals of the histidine-tagged-HupR protein, a transcriptional regulator from the photosynthetic bacterium Rhodobacter capsulatus, were obtained by specific interaction with a Ni(2+)-chelated lipid monolayer. HupR is a response regulator of the NtrC subfamily; i...
Main Authors: | Vénien-Bryan, C, Schertler, G, Thouvenin, E, Courty, S |
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格式: | Journal article |
语言: | English |
出版: |
2000
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