Selection of complementary single-variable domains for building monoclonal antibodies to native proteins.
Antibodies are now indispensable tools for all areas of cell biology and biotechnology as well as for diagnosis and therapy. Antigen-specific single immunoglobulin variable domains that bind to native antigens can be isolated and manipulated using yeast intracellular antibody capture technology but...
| Váldodahkkit: | , |
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| Materiálatiipa: | Journal article |
| Giella: | English |
| Almmustuhtton: |
2009
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| _version_ | 1826284745536831488 |
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| author | Tanaka, T Rabbitts, T |
| author_facet | Tanaka, T Rabbitts, T |
| author_sort | Tanaka, T |
| collection | OXFORD |
| description | Antibodies are now indispensable tools for all areas of cell biology and biotechnology as well as for diagnosis and therapy. Antigen-specific single immunoglobulin variable domains that bind to native antigens can be isolated and manipulated using yeast intracellular antibody capture technology but converting these to whole monoclonal antibody requires that complementary variable domains (VH or VL) bind to the same antigenic site. We describe a simple approach (CatcherAb) for specific isolation of such complementary single domains allowing the constitution of functional Fv, forming the basis of antigen-specific whole immunoglobulin and thus antibody production. We illustrate this approach by developing high-affinity Fv from single variable domains binding to RAS and LMO2 oncogenic proteins. |
| first_indexed | 2024-03-07T01:18:27Z |
| format | Journal article |
| id | oxford-uuid:8f830ab0-9e2b-4bad-9518-4394044e8847 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:18:27Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:8f830ab0-9e2b-4bad-9518-4394044e88472022-03-26T23:04:50ZSelection of complementary single-variable domains for building monoclonal antibodies to native proteins.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:8f830ab0-9e2b-4bad-9518-4394044e8847EnglishSymplectic Elements at Oxford2009Tanaka, TRabbitts, TAntibodies are now indispensable tools for all areas of cell biology and biotechnology as well as for diagnosis and therapy. Antigen-specific single immunoglobulin variable domains that bind to native antigens can be isolated and manipulated using yeast intracellular antibody capture technology but converting these to whole monoclonal antibody requires that complementary variable domains (VH or VL) bind to the same antigenic site. We describe a simple approach (CatcherAb) for specific isolation of such complementary single domains allowing the constitution of functional Fv, forming the basis of antigen-specific whole immunoglobulin and thus antibody production. We illustrate this approach by developing high-affinity Fv from single variable domains binding to RAS and LMO2 oncogenic proteins. |
| spellingShingle | Tanaka, T Rabbitts, T Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title | Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title_full | Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title_fullStr | Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title_full_unstemmed | Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title_short | Selection of complementary single-variable domains for building monoclonal antibodies to native proteins. |
| title_sort | selection of complementary single variable domains for building monoclonal antibodies to native proteins |
| work_keys_str_mv | AT tanakat selectionofcomplementarysinglevariabledomainsforbuildingmonoclonalantibodiestonativeproteins AT rabbittst selectionofcomplementarysinglevariabledomainsforbuildingmonoclonalantibodiestonativeproteins |