The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis
Ribosome biogenesis is an essential process in all living cells, which entails countless highly sequential and dynamic structural reorganization events. These include formation of dozens RNA helices through Watson-Crick base-pairing within ribosomal RNAs (rRNAs) and between rRNAs and small nucleolar...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2019
|
| _version_ | 1797081899187830784 |
|---|---|
| author | Roychowdhury, A Joret, C Bourgeois, G Heurgué-Hamard, V Lafontaine, D Graille, M |
| author_facet | Roychowdhury, A Joret, C Bourgeois, G Heurgué-Hamard, V Lafontaine, D Graille, M |
| author_sort | Roychowdhury, A |
| collection | OXFORD |
| description | Ribosome biogenesis is an essential process in all living cells, which entails countless highly sequential and dynamic structural reorganization events. These include formation of dozens RNA helices through Watson-Crick base-pairing within ribosomal RNAs (rRNAs) and between rRNAs and small nucleolar RNAs (snoRNAs), transient association of hundreds of proteinaceous assembly factors to nascent precursor (pre-)ribosomes, and stable assembly of ribosomal proteins. Unsurprisingly, the largest group of ribosome assembly factors are energy-consuming proteins (NTPases) including 25 RNA helicases in budding yeast. Among these, the DEAH-box Dhr1 is essential to displace the box C/D snoRNA U3 from the pre-rRNAs where it is bound in order to prevent premature formation of the central pseudoknot, a dramatic irreversible long-range interaction essential to the overall folding of the small ribosomal subunit. Here, we report the crystal structure of the Dhr1 helicase module, revealing the presence of a remarkable carboxyl-terminal domain essential for Dhr1 function in ribosome biogenesis in vivo and important for its interaction with its coactivator Utp14 in vitro. Furthermore, we report the functional consequences on ribosome biogenesis of DHX37 (human Dhr1) mutations found in patients suffering from microcephaly and other neurological diseases. |
| first_indexed | 2024-03-07T01:20:32Z |
| format | Journal article |
| id | oxford-uuid:90299163-27fb-49bd-af22-17dc0d278e42 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:20:32Z |
| publishDate | 2019 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:90299163-27fb-49bd-af22-17dc0d278e422022-03-26T23:09:44ZThe DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:90299163-27fb-49bd-af22-17dc0d278e42EnglishSymplectic Elements at OxfordOxford University Press2019Roychowdhury, AJoret, CBourgeois, GHeurgué-Hamard, VLafontaine, DGraille, MRibosome biogenesis is an essential process in all living cells, which entails countless highly sequential and dynamic structural reorganization events. These include formation of dozens RNA helices through Watson-Crick base-pairing within ribosomal RNAs (rRNAs) and between rRNAs and small nucleolar RNAs (snoRNAs), transient association of hundreds of proteinaceous assembly factors to nascent precursor (pre-)ribosomes, and stable assembly of ribosomal proteins. Unsurprisingly, the largest group of ribosome assembly factors are energy-consuming proteins (NTPases) including 25 RNA helicases in budding yeast. Among these, the DEAH-box Dhr1 is essential to displace the box C/D snoRNA U3 from the pre-rRNAs where it is bound in order to prevent premature formation of the central pseudoknot, a dramatic irreversible long-range interaction essential to the overall folding of the small ribosomal subunit. Here, we report the crystal structure of the Dhr1 helicase module, revealing the presence of a remarkable carboxyl-terminal domain essential for Dhr1 function in ribosome biogenesis in vivo and important for its interaction with its coactivator Utp14 in vitro. Furthermore, we report the functional consequences on ribosome biogenesis of DHX37 (human Dhr1) mutations found in patients suffering from microcephaly and other neurological diseases. |
| spellingShingle | Roychowdhury, A Joret, C Bourgeois, G Heurgué-Hamard, V Lafontaine, D Graille, M The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title | The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title_full | The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title_fullStr | The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title_full_unstemmed | The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title_short | The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| title_sort | deah box rna helicase dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis |
| work_keys_str_mv | AT roychowdhurya thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT joretc thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT bourgeoisg thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT heurguehamardv thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT lafontained thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT graillem thedeahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT roychowdhurya deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT joretc deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT bourgeoisg deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT heurguehamardv deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT lafontained deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis AT graillem deahboxrnahelicasedhr1containsaremarkablecarboxylterminaldomainessentialforsmallribosomalsubunitbiogenesis |