Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia)
The Phyllidiidae (Gastropoda, Heterobranchia, Nudibranchia) is a family of colourful nudibranchs found on Indo-Pacific coral reefs. Despite the abundant and widespread occurrence of many species, their phylogenetic relationships are not well known. The present study is the first contribution to fill...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
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Pensoft Publishers
2016
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| _version_ | 1797082004989149184 |
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| author | van der Meij, S Stoffels, B Hoeksema, B van Alphen, J van Alen, T Meyers-Muñoz, M De Voogd, N Tuti, Y van der Velde, G |
| author_facet | van der Meij, S Stoffels, B Hoeksema, B van Alphen, J van Alen, T Meyers-Muñoz, M De Voogd, N Tuti, Y van der Velde, G |
| author_sort | van der Meij, S |
| collection | OXFORD |
| description | The Phyllidiidae (Gastropoda, Heterobranchia, Nudibranchia) is a family of colourful nudibranchs found on Indo-Pacific coral reefs. Despite the abundant and widespread occurrence of many species, their phylogenetic relationships are not well known. The present study is the first contribution to fill the gap in our knowledge on their phylogeny by combining morphological and molecular data. For that purpose 99 specimens belonging to 16 species were collected at two localities in Indonesia. They were photographed and used to make a phylogeny reconstruction based on newly obtained cytochrome oxidase subunit (COI) sequences as well as sequence data from GenBank. All mitochondrial 16S sequence data available from GenBank were used in a separate phylogeny reconstruction to obtain information for species we did not collect. COI data allowed the distinction of the genera and species, whereas the 16S data gave a mixed result with respect to the genera Phyllidia and Phyllidiella. Specimens which could be ascribed to species level based on their external morphology and colour patterns showed low variation in COI sequences, but there were two exceptions: three specimens identified as Phyllidia cf. babai represent two to three different species, while Phyllidiella pustulosa showed highly supported subclades. The barcoding marker COI also confirms that the species boundaries in morphologically highly variable species such as Phyllidia elegans, P. varicosa, and Phyllidiopsis krempfi, are correct as presently understood. In the COI as well as the 16S cladogram Phyllidiopsis cardinalis was located separately from all other Phyllidiidae, whereas Phyllidiopsis fissuratus was positioned alone from the Phyllidiella species by COI data only. Future studies on phyllidiid systematics should continue to combine morphological information with DNA sequences to obtain a clearer insight in their phylogeny. |
| first_indexed | 2024-03-07T01:22:02Z |
| format | Journal article |
| id | oxford-uuid:90b06277-8690-4333-be7f-5a955994d439 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:22:02Z |
| publishDate | 2016 |
| publisher | Pensoft Publishers |
| record_format | dspace |
| spelling | oxford-uuid:90b06277-8690-4333-be7f-5a955994d4392022-03-26T23:13:27ZPhylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia)Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:90b06277-8690-4333-be7f-5a955994d439Symplectic Elements at OxfordPensoft Publishers2016van der Meij, SStoffels, BHoeksema, Bvan Alphen, Jvan Alen, TMeyers-Muñoz, MDe Voogd, NTuti, Yvan der Velde, GThe Phyllidiidae (Gastropoda, Heterobranchia, Nudibranchia) is a family of colourful nudibranchs found on Indo-Pacific coral reefs. Despite the abundant and widespread occurrence of many species, their phylogenetic relationships are not well known. The present study is the first contribution to fill the gap in our knowledge on their phylogeny by combining morphological and molecular data. For that purpose 99 specimens belonging to 16 species were collected at two localities in Indonesia. They were photographed and used to make a phylogeny reconstruction based on newly obtained cytochrome oxidase subunit (COI) sequences as well as sequence data from GenBank. All mitochondrial 16S sequence data available from GenBank were used in a separate phylogeny reconstruction to obtain information for species we did not collect. COI data allowed the distinction of the genera and species, whereas the 16S data gave a mixed result with respect to the genera Phyllidia and Phyllidiella. Specimens which could be ascribed to species level based on their external morphology and colour patterns showed low variation in COI sequences, but there were two exceptions: three specimens identified as Phyllidia cf. babai represent two to three different species, while Phyllidiella pustulosa showed highly supported subclades. The barcoding marker COI also confirms that the species boundaries in morphologically highly variable species such as Phyllidia elegans, P. varicosa, and Phyllidiopsis krempfi, are correct as presently understood. In the COI as well as the 16S cladogram Phyllidiopsis cardinalis was located separately from all other Phyllidiidae, whereas Phyllidiopsis fissuratus was positioned alone from the Phyllidiella species by COI data only. Future studies on phyllidiid systematics should continue to combine morphological information with DNA sequences to obtain a clearer insight in their phylogeny. |
| spellingShingle | van der Meij, S Stoffels, B Hoeksema, B van Alphen, J van Alen, T Meyers-Muñoz, M De Voogd, N Tuti, Y van der Velde, G Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title | Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title_full | Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title_fullStr | Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title_full_unstemmed | Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title_short | Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia) |
| title_sort | phylogenetic relationships within the phyllidiidae opisthobranchia nudibranchia |
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