The proteomes of transcription factories containing RNA polymerases I, II or III.
Human nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammali...
| المؤلفون الرئيسيون: | , , , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2011
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| _version_ | 1826285086659575808 |
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| author | Melnik, S Deng, B Papantonis, A Baboo, S Carr, I Cook, P |
| author_facet | Melnik, S Deng, B Papantonis, A Baboo, S Carr, I Cook, P |
| author_sort | Melnik, S |
| collection | OXFORD |
| description | Human nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammalian cells. After isolation, we analyzed their protein content by mass spectrometry. Each complex represents part of the core of a transcription factory. For example, the RNA polymerase II complex contains subunits unique to RNA polymerase II plus various transcription factors but shares a number of ribonucleoproteins with the other polymerase complexes; it is also rich in polymerase II transcripts. We also describe a native chromosome conformation capture method to confirm that the complexes remain attached to the same pairs of DNA templates found in vivo. |
| first_indexed | 2024-03-07T01:23:35Z |
| format | Journal article |
| id | oxford-uuid:912d0f6b-7885-4305-abb7-4447e86c9fc7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:23:35Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:912d0f6b-7885-4305-abb7-4447e86c9fc72022-03-26T23:16:59ZThe proteomes of transcription factories containing RNA polymerases I, II or III.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:912d0f6b-7885-4305-abb7-4447e86c9fc7EnglishSymplectic Elements at Oxford2011Melnik, SDeng, BPapantonis, ABaboo, SCarr, ICook, PHuman nuclei contain three RNA polymerases (I, II and III) that transcribe different groups of genes; the active forms of all three are difficult to isolate because they are bound to the substructure. Here we describe a purification approach for isolating active RNA polymerase complexes from mammalian cells. After isolation, we analyzed their protein content by mass spectrometry. Each complex represents part of the core of a transcription factory. For example, the RNA polymerase II complex contains subunits unique to RNA polymerase II plus various transcription factors but shares a number of ribonucleoproteins with the other polymerase complexes; it is also rich in polymerase II transcripts. We also describe a native chromosome conformation capture method to confirm that the complexes remain attached to the same pairs of DNA templates found in vivo. |
| spellingShingle | Melnik, S Deng, B Papantonis, A Baboo, S Carr, I Cook, P The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title | The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title_full | The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title_fullStr | The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title_full_unstemmed | The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title_short | The proteomes of transcription factories containing RNA polymerases I, II or III. |
| title_sort | proteomes of transcription factories containing rna polymerases i ii or iii |
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