The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man.
The cytosolic Ca2+ activity was measured with the fluorescent indicator quin-2 in pancreatic beta-cells obtained from obese-hyperglycemic mice. When present at a concentration of 20 mmol/l in a medium physiologically balanced in cations, glucose induced a rise of cytosolic Ca2+ after a delay of 1--3...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1985
|
| _version_ | 1797082158358069248 |
|---|---|
| author | Hellman, B Hällgren, R Abrahamsson, H Bergsten, P Berne, C Gylfe, E Rorsman, P Wide, L |
| author_facet | Hellman, B Hällgren, R Abrahamsson, H Bergsten, P Berne, C Gylfe, E Rorsman, P Wide, L |
| author_sort | Hellman, B |
| collection | OXFORD |
| description | The cytosolic Ca2+ activity was measured with the fluorescent indicator quin-2 in pancreatic beta-cells obtained from obese-hyperglycemic mice. When present at a concentration of 20 mmol/l in a medium physiologically balanced in cations, glucose induced a rise of cytosolic Ca2+ after a delay of 1--3 min. At lower concentrations of extracellular Ca2+ this effect was not only prevented but the sugar promptly reduced the cytosolic Ca2+ activity. The dual effect of glucose on cytosolic Ca2+ had its counterpart in the release of insulin. Whereas 20 mmol/l of glucose stimulated the release of insulin from mouse islets previously stored in a Ca2+-deficient medium, the sugar was clearly inhibitory when present at a concentration of 6 mmol/l. Intravenous glucose tolerance tests revealed a temporary glucose depression of the serum concentrations of insulin and C-peptide in several patients with diabetes. In a mentally retarded girl with hyperinsulinemia associated with acanthosis nigricans the glucose suppression of circulating insulin was prolonged and sufficiently pronounced to suggest an almost complete inhibition of the secretory activity of the pancreatic B-cells. |
| first_indexed | 2024-03-07T01:24:13Z |
| format | Journal article |
| id | oxford-uuid:9167ced2-37d1-4b44-b3a7-cc363f2a7ef6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:24:13Z |
| publishDate | 1985 |
| record_format | dspace |
| spelling | oxford-uuid:9167ced2-37d1-4b44-b3a7-cc363f2a7ef62022-03-26T23:18:32ZThe dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9167ced2-37d1-4b44-b3a7-cc363f2a7ef6EnglishSymplectic Elements at Oxford1985Hellman, BHällgren, RAbrahamsson, HBergsten, PBerne, CGylfe, ERorsman, PWide, LThe cytosolic Ca2+ activity was measured with the fluorescent indicator quin-2 in pancreatic beta-cells obtained from obese-hyperglycemic mice. When present at a concentration of 20 mmol/l in a medium physiologically balanced in cations, glucose induced a rise of cytosolic Ca2+ after a delay of 1--3 min. At lower concentrations of extracellular Ca2+ this effect was not only prevented but the sugar promptly reduced the cytosolic Ca2+ activity. The dual effect of glucose on cytosolic Ca2+ had its counterpart in the release of insulin. Whereas 20 mmol/l of glucose stimulated the release of insulin from mouse islets previously stored in a Ca2+-deficient medium, the sugar was clearly inhibitory when present at a concentration of 6 mmol/l. Intravenous glucose tolerance tests revealed a temporary glucose depression of the serum concentrations of insulin and C-peptide in several patients with diabetes. In a mentally retarded girl with hyperinsulinemia associated with acanthosis nigricans the glucose suppression of circulating insulin was prolonged and sufficiently pronounced to suggest an almost complete inhibition of the secretory activity of the pancreatic B-cells. |
| spellingShingle | Hellman, B Hällgren, R Abrahamsson, H Bergsten, P Berne, C Gylfe, E Rorsman, P Wide, L The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title | The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title_full | The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title_fullStr | The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title_full_unstemmed | The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title_short | The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man. |
| title_sort | dual action of glucose on the cytosolic ca2 activity in pancreatic beta cells demonstration of an inhibitory effect of glucose on insulin release in the mouse and man |
| work_keys_str_mv | AT hellmanb thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT hallgrenr thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT abrahamssonh thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT bergstenp thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT bernec thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT gylfee thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT rorsmanp thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT widel thedualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT hellmanb dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT hallgrenr dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT abrahamssonh dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT bergstenp dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT bernec dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT gylfee dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT rorsmanp dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman AT widel dualactionofglucoseonthecytosolicca2activityinpancreaticbetacellsdemonstrationofaninhibitoryeffectofglucoseoninsulinreleaseinthemouseandman |