PROTOPLAST FORMATION AND REGENERATION FROM SPORANGIA AND ENCYSTED ZOOSPORES OF PHYTOPHTHORA-INFESTANS
Protoplasts of Phytophthora infestans were formed from sporangia or encysted zoospores by digestion with NovoZym 234 in stabilizing osmotica. The highest yields, representing a conversion of up to 90% of initial sporangia or cysts were obtained in solKCl CaCl2 mixtures with osmolalities around 1·7 o...
Main Authors: | Campbell, A, Moon, R, Duncan, J, Gurr, S, Kinghorn, J |
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Format: | Journal article |
Language: | English |
Published: |
1989
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