Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy.
Deuterium nuclear magnetic resonance (NMR) spectroscopy was used to study the partitioning behaviour of 1-hexanol specifically deuterated in the alpha-position into model lipid bilayers. In all systems studied, the observed deuterium NMR lineshapes were time-dependent. Initially, 1-hexanol-d2 gave r...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1797082196469612544 |
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| author | Fraser, D Van Gorkom, L Watts, A |
| author_facet | Fraser, D Van Gorkom, L Watts, A |
| author_sort | Fraser, D |
| collection | OXFORD |
| description | Deuterium nuclear magnetic resonance (NMR) spectroscopy was used to study the partitioning behaviour of 1-hexanol specifically deuterated in the alpha-position into model lipid bilayers. In all systems studied, the observed deuterium NMR lineshapes were time-dependent. Initially, 1-hexanol-d2 gave rise to an isotropic deuterium resonance with a different chemical shift from that of aqueous 1-hexanol-d2. After equilibration over a period of days, a broader spectral component characteristic of a spherically-averaged powder-pattern was observed. The quadrupole anisotropy of the 1-hexanol-d2 giving rise to the broad spectrum depended upon the cholesterol content of the membrane. From quantitation of the anisotropic to isotropic deuterium NMR spectra, the partition coefficients of 1-hexanol-d2 in a number of bilayer systems (asolectin and phosphatidylcholine bilayers (the latter with and without cholesterol] were determined. The partitioning of 1-hexanol-d2 into red blood cell membranes, and a suspension of lipids extracted from red blood cell membranes, was also examined. It is suggested that 1-hexanol, and probably other lipophiles, can partition to either the bilayer surface or the bilayer interior in a time-dependent manner. |
| first_indexed | 2024-03-07T01:24:47Z |
| format | Journal article |
| id | oxford-uuid:9199864f-0400-4b68-adb1-e10d35946143 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:24:47Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:9199864f-0400-4b68-adb1-e10d359461432022-03-26T23:19:46ZPartitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9199864f-0400-4b68-adb1-e10d35946143EnglishSymplectic Elements at Oxford1991Fraser, DVan Gorkom, LWatts, ADeuterium nuclear magnetic resonance (NMR) spectroscopy was used to study the partitioning behaviour of 1-hexanol specifically deuterated in the alpha-position into model lipid bilayers. In all systems studied, the observed deuterium NMR lineshapes were time-dependent. Initially, 1-hexanol-d2 gave rise to an isotropic deuterium resonance with a different chemical shift from that of aqueous 1-hexanol-d2. After equilibration over a period of days, a broader spectral component characteristic of a spherically-averaged powder-pattern was observed. The quadrupole anisotropy of the 1-hexanol-d2 giving rise to the broad spectrum depended upon the cholesterol content of the membrane. From quantitation of the anisotropic to isotropic deuterium NMR spectra, the partition coefficients of 1-hexanol-d2 in a number of bilayer systems (asolectin and phosphatidylcholine bilayers (the latter with and without cholesterol] were determined. The partitioning of 1-hexanol-d2 into red blood cell membranes, and a suspension of lipids extracted from red blood cell membranes, was also examined. It is suggested that 1-hexanol, and probably other lipophiles, can partition to either the bilayer surface or the bilayer interior in a time-dependent manner. |
| spellingShingle | Fraser, D Van Gorkom, L Watts, A Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title | Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title_full | Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title_fullStr | Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title_full_unstemmed | Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title_short | Partitioning behaviour of 1-hexanol into lipid membranes as studied by deuterium NMR spectroscopy. |
| title_sort | partitioning behaviour of 1 hexanol into lipid membranes as studied by deuterium nmr spectroscopy |
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