A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells
Aryl boronate fluorescent probes allow the non-invasive study of dynamic cellular processes involving the reactive species, hydrogen peroxide (H2O2) and peroxynitrite (ONOO−). However, the ability of these probes to differentiate between these two species remains unclear. Here, we report a boronate-...
| المؤلفون الرئيسيون: | , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
Royal Society of Chemistry
2022
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| _version_ | 1826308885948923904 |
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| author | Bolland, HR Hammond, EM Sedgwick, AC |
| author_facet | Bolland, HR Hammond, EM Sedgwick, AC |
| author_sort | Bolland, HR |
| collection | OXFORD |
| description | Aryl boronate fluorescent probes allow the non-invasive study of dynamic cellular processes involving the reactive species, hydrogen peroxide (H2O2) and peroxynitrite (ONOO−). However, the ability of these probes to differentiate between these two species remains unclear. Here, we report a boronate-functionalised hemicyanine dye (<strong>HD-BPin</strong>) as a potential strategy to distinguish between H2O2 at 704 nm (red channel) and ONOO− at 460 nm (blue channel) in solution and in cells. This work also highlights the choice of fluorophore before boronate functionalization can dictate the observed selectivity between these two species. |
| first_indexed | 2024-03-07T07:25:56Z |
| format | Journal article |
| id | oxford-uuid:91be89cf-dcb6-49a9-bc83-83d6368d3a2e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:25:56Z |
| publishDate | 2022 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:91be89cf-dcb6-49a9-bc83-83d6368d3a2e2022-11-18T10:40:32ZA fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:91be89cf-dcb6-49a9-bc83-83d6368d3a2eEnglishSymplectic ElementsRoyal Society of Chemistry2022Bolland, HRHammond, EMSedgwick, ACAryl boronate fluorescent probes allow the non-invasive study of dynamic cellular processes involving the reactive species, hydrogen peroxide (H2O2) and peroxynitrite (ONOO−). However, the ability of these probes to differentiate between these two species remains unclear. Here, we report a boronate-functionalised hemicyanine dye (<strong>HD-BPin</strong>) as a potential strategy to distinguish between H2O2 at 704 nm (red channel) and ONOO− at 460 nm (blue channel) in solution and in cells. This work also highlights the choice of fluorophore before boronate functionalization can dictate the observed selectivity between these two species. |
| spellingShingle | Bolland, HR Hammond, EM Sedgwick, AC A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title | A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title_full | A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title_fullStr | A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title_full_unstemmed | A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title_short | A fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
| title_sort | fluorescent probe strategy for the detection and discrimination of hydrogen peroxide and peroxynitrite in cells |
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