Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity.
The presence of both cell-mediated and humoral immunity is important in protection from and clearance of a number of infectious pathogens. We describe novel vaccine regimens using combinations of plasmid DNA, poxvirus and protein to induce strong Ag-specific T cell and Ab responses simultaneously in...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2005
|
| _version_ | 1797082256829841408 |
|---|---|
| author | Hutchings, C Gilbert, S Hill, A Moore, A |
| author_facet | Hutchings, C Gilbert, S Hill, A Moore, A |
| author_sort | Hutchings, C |
| collection | OXFORD |
| description | The presence of both cell-mediated and humoral immunity is important in protection from and clearance of a number of infectious pathogens. We describe novel vaccine regimens using combinations of plasmid DNA, poxvirus and protein to induce strong Ag-specific T cell and Ab responses simultaneously in a murine model. Intramuscular (i.m.) immunization with plasmid DNA encoding the middle Ag of hepatitis B (DNA) concurrently with a commercial hepatitis B virus (HBV) vaccine (Engerix-B) followed by boosting immunizations with both modified vaccinia virus Ankara (MVA) encoding the middle Ag of HBV and Engerix-B induced high levels of CD4(+) and CD8(+) T cells and high titer Ab responses to hepatitis B surface Ag (HbsAg). Substitution of Engerix-B with adjuvant-free rHBsAg induced similar T cell responses and greatly enhanced Ab levels. Repeated immunizations with recombinant or nonrecombinant MVA mixed with Ag induced higher titers of Abs compared with immunization with either Ag or Engerix-B further demonstrating this novel adjuvant effect of MVA. The poxviruses NYVAC, fowlpox (FP9) and ALVAC, and to a lesser extent, adenovirus, also displayed similar adjuvant properties when used in combination with rHBsAg. The use of poxviruses as an adjuvant for protein to concurrently induce Ag-specific T cells and Abs could be applied to the development of vaccines for many diseases, including HIV and malaria, where both cell mediated and humoral immunity may be important for protection. |
| first_indexed | 2024-03-07T01:25:33Z |
| format | Journal article |
| id | oxford-uuid:91dbe4ab-28c2-43cc-bedc-1aa15591d914 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:25:33Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:91dbe4ab-28c2-43cc-bedc-1aa15591d9142022-03-26T23:21:28ZNovel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:91dbe4ab-28c2-43cc-bedc-1aa15591d914EnglishSymplectic Elements at Oxford2005Hutchings, CGilbert, SHill, AMoore, AThe presence of both cell-mediated and humoral immunity is important in protection from and clearance of a number of infectious pathogens. We describe novel vaccine regimens using combinations of plasmid DNA, poxvirus and protein to induce strong Ag-specific T cell and Ab responses simultaneously in a murine model. Intramuscular (i.m.) immunization with plasmid DNA encoding the middle Ag of hepatitis B (DNA) concurrently with a commercial hepatitis B virus (HBV) vaccine (Engerix-B) followed by boosting immunizations with both modified vaccinia virus Ankara (MVA) encoding the middle Ag of HBV and Engerix-B induced high levels of CD4(+) and CD8(+) T cells and high titer Ab responses to hepatitis B surface Ag (HbsAg). Substitution of Engerix-B with adjuvant-free rHBsAg induced similar T cell responses and greatly enhanced Ab levels. Repeated immunizations with recombinant or nonrecombinant MVA mixed with Ag induced higher titers of Abs compared with immunization with either Ag or Engerix-B further demonstrating this novel adjuvant effect of MVA. The poxviruses NYVAC, fowlpox (FP9) and ALVAC, and to a lesser extent, adenovirus, also displayed similar adjuvant properties when used in combination with rHBsAg. The use of poxviruses as an adjuvant for protein to concurrently induce Ag-specific T cells and Abs could be applied to the development of vaccines for many diseases, including HIV and malaria, where both cell mediated and humoral immunity may be important for protection. |
| spellingShingle | Hutchings, C Gilbert, S Hill, A Moore, A Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title | Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title_full | Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title_fullStr | Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title_full_unstemmed | Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title_short | Novel protein and poxvirus-based vaccine combinations for simultaneous induction of humoral and cell-mediated immunity. |
| title_sort | novel protein and poxvirus based vaccine combinations for simultaneous induction of humoral and cell mediated immunity |
| work_keys_str_mv | AT hutchingsc novelproteinandpoxvirusbasedvaccinecombinationsforsimultaneousinductionofhumoralandcellmediatedimmunity AT gilberts novelproteinandpoxvirusbasedvaccinecombinationsforsimultaneousinductionofhumoralandcellmediatedimmunity AT hilla novelproteinandpoxvirusbasedvaccinecombinationsforsimultaneousinductionofhumoralandcellmediatedimmunity AT moorea novelproteinandpoxvirusbasedvaccinecombinationsforsimultaneousinductionofhumoralandcellmediatedimmunity |