$Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.$

Crystallization and preliminary X-ray diffraction studies on a recombinant isopenicillin N synthase from Cephalosporium acremonium.

Recombinant isopenicillin N synthase from Cephalosporium acremonium was expressed in Escherichia coli and the protein was purified. After nearly 5000 crystallization trials, the apo enzyme was crystallized by the hanging drop vapour diffusion technique, using polyethylene glycol and lithium sulphate...

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Bibliografiske detaljer
Main Authors:	Fujishima, Y, Nordlund, P, Pelosi, G, Schofield, C, Cole, S, Baldwin, J, Hajdu, J
Format:	Journal article
Sprog:	English
Udgivet:	1994

Lignende værker

Crystallization and preliminary X-ray diffraction studies on recombinant isopenicillin N synthase from Aspergillus nidulans.
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Udgivet: (1995)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus.
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Udgivet: (1990)

EXAFS STUDIES OF ISOPENICILLIN-N SYNTHASE
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Udgivet: (1992)

Molecular weight analysis of isopenicillin N synthase by electrospray mass spectrometry.
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Udgivet: (1990)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
af: Baldwin, J, et al.
Udgivet: (1991)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
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Udgivet: (1992)

X-ray absorption studies of the ferrous active site of isopenicillin N synthase and related model complexes.
af: Randall, C, et al.
Udgivet: (1993)

Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase.
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Udgivet: (1991)

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.
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Udgivet: (1997)

Conversion of penicillin N to cephalosporin(s) by cell-free extracts of cephalosporium acremonium /
af: 350976 Kohsaka, M., et al.

Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.
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Udgivet: (1996)

Homologous transformation of Cephalosporium acremonium with the nitrate reductase-encoding gene (niaD).
af: Whitehead, M, et al.
Udgivet: (1990)

FORMATION OF A NOVEL BICYCLIC GAMMA-LACTAM WITH ISOPENICILLIN-N SYNTHASE
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Udgivet: (1989)

Substrate specificity of L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase from Cephalosporium acremonium: demonstration of the structure of several unnatural tripeptide products.
af: Baldwin, J, et al.
Udgivet: (1994)

GAMMA-LACTAM FORMATION FROM TRIPEPTIDES WITH ISOPENICILLIN-N SYNTHASE
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Udgivet: (1988)

Formation and properties of protoplasts from antibiotic-producing strains of penicillium chrysogenum and cephalosporium acremonium /

Glutamine-330 is not essential for activity in isopenicillin N synthase from Aspergillus nidulans.
af: Sami, M, et al.
Udgivet: (1997)

Anaerobic crystallisation of an isopenicillin N synthase center dot Fe(II)center dot substrate complex demonstrated by X-ray studies
af: Roach, P, et al.
Udgivet: (1996)

Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes.
af: Roach, P, et al.
Udgivet: (1995)

Studies on side chain interactions during the isopenicillin N synthase catalysed biosynthesis of penicillins
af: Cavelier, F, et al.
Udgivet: (1997)

Studies of isopenicillin N synthase enzymatic properties using a continuous spectrophotometric assay.
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Udgivet: (2000)

Mechanistic studies on isopenicillin n synthase
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Udgivet: (1991)

The crystal structure of isopenicillin N synthase with a dipeptide substrate analogue.
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Udgivet: (2013)

Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in ß-lactam biosynthesis
af: Rabe, P, et al.
Udgivet: (2018)

The crystal structure of an LLL-configured depsipeptide substrate analogue bound to isopenicillin N synthase.
af: Ge, W, et al.
Udgivet: (2010)

Purification, crystallization and preliminary X-ray diffraction of anthocyanidin synthase from Arabidopsis thaliana.
af: Turnbull, J, et al.
Udgivet: (2001)

Modification of iron binding ligands in isopenicillin n synthase
af: Sami, M, et al.
Udgivet: (1998)

Crystallographic studies on the binding of selectively deuterated LLD- and LLL-substrate epimers by isopenicillin N synthase.
af: Ge, W, et al.
Udgivet: (2010)

Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.
af: Long, A, et al.
Udgivet: (2003)

X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis
af: Rabe, P, et al.
Udgivet: (2021)

Structural studies on the reaction of isopenicillin N synthase with a sterically demanding depsipeptide substrate analogue.
af: Ge, W, et al.
Udgivet: (2009)

Photoaffinity labelling of isopenicillin N synthetase.
af: Baldwin, J, et al.
Udgivet: (1990)

Taxonomic criteria in the genus Cephalosporium
af: Walter Gams
Udgivet: (2014-11-01)

Occurrence of Isopenicillin-N-Synthase Homologs in Bioluminescent Ctenophores and Implications for Coelenterazine Biosynthesis.
af: Warren R Francis, et al.
Udgivet: (2015-01-01)

Production of functionally active <it>Penicillium chrysogenum </it>isopenicillin N synthase in the yeast <it>Hansenula polymorpha</it>
af: Veenhuis Marten, et al.
Udgivet: (2008-03-01)

The crystal structure of isopenicillin N synthase with δ-((L)-α-aminoadipoyl)-(L)-cysteinyl-(D)-methionine reveals thioether coordination to iron.
af: Clifton, I, et al.
Udgivet: (2011)

Photoaffinity labelling of isopenicillin N synthetase by laser-flash photolysis.
af: Baldwin, J, et al.
Udgivet: (1989)

Spectroscopic studies reveal details of substrate-induced conformational changes distant from the active site in isopenicillin N synthase
af: Rabe, P, et al.
Udgivet: (2022)

Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine.
af: Long, A, et al.
Udgivet: (2005)

X-ray absorption spectroscopic studies of the iron(II) active site of clavaminic acid synthase.
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Udgivet: (1997)