Store-operated cyclic AMP signalling mediated by STIM1.
Depletion of Ca(2+) from the endoplasmic reticulum (ER) results in activation of plasma membrane Ca(2+) entry channels. This 'store-operated' process requires translocation of a transmembrane ER Ca(2+) sensor protein, stromal interaction molecule 1 (STIM1), to sites closely apposed to Ca(2...
| Hlavní autoři: | , , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2009
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| _version_ | 1826285245273473024 |
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| author | Lefkimmiatis, K Srikanthan, M Maiellaro, I Moyer, M Curci, S Hofer, A |
| author_facet | Lefkimmiatis, K Srikanthan, M Maiellaro, I Moyer, M Curci, S Hofer, A |
| author_sort | Lefkimmiatis, K |
| collection | OXFORD |
| description | Depletion of Ca(2+) from the endoplasmic reticulum (ER) results in activation of plasma membrane Ca(2+) entry channels. This 'store-operated' process requires translocation of a transmembrane ER Ca(2+) sensor protein, stromal interaction molecule 1 (STIM1), to sites closely apposed to Ca(2+) channels at the cell surface. However, it is not known whether a reduction in Ca(2+) stores is coupled to other signalling pathways by this mechanism. We found that lowering the concentration of free Ca(2+) in the ER, independently of the cytosolic Ca(2+) concentration, also led to recruitment of adenylyl cyclases. This resulted in enhanced cAMP accumulation and PKA activation, measured using FRET-based cAMP indicators. Translocation of STIM1 was required for efficient coupling of ER Ca(2+) depletion to adenylyl cyclase activity. We propose the existence of a pathway (store-operated cAMP signalling or SOcAMPS) in which the content of internal Ca(2+) stores is directly connected to cAMP signalling through a process that involves STIM1. |
| first_indexed | 2024-03-07T01:25:57Z |
| format | Journal article |
| id | oxford-uuid:91fc28e2-04cc-4790-bee4-f0e7bc07f1f5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:25:57Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:91fc28e2-04cc-4790-bee4-f0e7bc07f1f52022-03-26T23:22:27ZStore-operated cyclic AMP signalling mediated by STIM1.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:91fc28e2-04cc-4790-bee4-f0e7bc07f1f5EnglishSymplectic Elements at Oxford2009Lefkimmiatis, KSrikanthan, MMaiellaro, IMoyer, MCurci, SHofer, ADepletion of Ca(2+) from the endoplasmic reticulum (ER) results in activation of plasma membrane Ca(2+) entry channels. This 'store-operated' process requires translocation of a transmembrane ER Ca(2+) sensor protein, stromal interaction molecule 1 (STIM1), to sites closely apposed to Ca(2+) channels at the cell surface. However, it is not known whether a reduction in Ca(2+) stores is coupled to other signalling pathways by this mechanism. We found that lowering the concentration of free Ca(2+) in the ER, independently of the cytosolic Ca(2+) concentration, also led to recruitment of adenylyl cyclases. This resulted in enhanced cAMP accumulation and PKA activation, measured using FRET-based cAMP indicators. Translocation of STIM1 was required for efficient coupling of ER Ca(2+) depletion to adenylyl cyclase activity. We propose the existence of a pathway (store-operated cAMP signalling or SOcAMPS) in which the content of internal Ca(2+) stores is directly connected to cAMP signalling through a process that involves STIM1. |
| spellingShingle | Lefkimmiatis, K Srikanthan, M Maiellaro, I Moyer, M Curci, S Hofer, A Store-operated cyclic AMP signalling mediated by STIM1. |
| title | Store-operated cyclic AMP signalling mediated by STIM1. |
| title_full | Store-operated cyclic AMP signalling mediated by STIM1. |
| title_fullStr | Store-operated cyclic AMP signalling mediated by STIM1. |
| title_full_unstemmed | Store-operated cyclic AMP signalling mediated by STIM1. |
| title_short | Store-operated cyclic AMP signalling mediated by STIM1. |
| title_sort | store operated cyclic amp signalling mediated by stim1 |
| work_keys_str_mv | AT lefkimmiatisk storeoperatedcyclicampsignallingmediatedbystim1 AT srikanthanm storeoperatedcyclicampsignallingmediatedbystim1 AT maiellaroi storeoperatedcyclicampsignallingmediatedbystim1 AT moyerm storeoperatedcyclicampsignallingmediatedbystim1 AT curcis storeoperatedcyclicampsignallingmediatedbystim1 AT hofera storeoperatedcyclicampsignallingmediatedbystim1 |