RNA-Seq dataset generated from stimulation of cultured Tc2 cells
The purpose of this data is to investigate the transcriptional response of type 2 CD8+ T cells to different modes of activation. Tc2 cells were isolated by FACS sorting CRTH2+ CD8+ T cells from cone PBMCs. They were expanded using anti-CD3 and IL-2 for 3 weeks in RPMI + 10% FCS. They were stimula...
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| Format: | Dataset |
| Language: | English |
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University of Oxford
2021
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| _version_ | 1826285372041068544 |
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| author | Hoyle, R |
| author2 | Hoyle, R |
| author_facet | Hoyle, R Hoyle, R |
| author_sort | Hoyle, R |
| collection | OXFORD |
| description | The purpose of this data is to investigate the transcriptional response of type 2 CD8+ T cells to different modes of activation.
Tc2 cells were isolated by FACS sorting CRTH2+ CD8+ T cells from cone PBMCs.
They were expanded using anti-CD3 and IL-2 for 3 weeks in RPMI + 10% FCS.
They were stimulated either with anti-CD3 + anti-CD28, PGD2, or a combination of alarmin cytokines (IL-25, IL-33, and TSLP).
Total RNA was isolated and analysed by RNA-seq.
This file includes the read counts, the results output of DESeq2 differential gene expression analysis, and the results of gene set enrichment analysis (GSEA). |
| first_indexed | 2024-03-07T01:27:49Z |
| format | Dataset |
| id | oxford-uuid:928fcb58-c7af-4219-b356-d941373a0334 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:27:49Z |
| publishDate | 2021 |
| publisher | University of Oxford |
| record_format | dspace |
| spelling | oxford-uuid:928fcb58-c7af-4219-b356-d941373a03342022-03-26T23:26:26ZRNA-Seq dataset generated from stimulation of cultured Tc2 cellsDatasethttp://purl.org/coar/resource_type/c_ddb1uuid:928fcb58-c7af-4219-b356-d941373a0334AsthmaAllergyImmunologyEnglishHyrax DepositUniversity of Oxford2021Hoyle, RHoyle, RXue, LThe purpose of this data is to investigate the transcriptional response of type 2 CD8+ T cells to different modes of activation. Tc2 cells were isolated by FACS sorting CRTH2+ CD8+ T cells from cone PBMCs. They were expanded using anti-CD3 and IL-2 for 3 weeks in RPMI + 10% FCS. They were stimulated either with anti-CD3 + anti-CD28, PGD2, or a combination of alarmin cytokines (IL-25, IL-33, and TSLP). Total RNA was isolated and analysed by RNA-seq. This file includes the read counts, the results output of DESeq2 differential gene expression analysis, and the results of gene set enrichment analysis (GSEA). |
| spellingShingle | Asthma Allergy Immunology Hoyle, R RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title | RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title_full | RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title_fullStr | RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title_full_unstemmed | RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title_short | RNA-Seq dataset generated from stimulation of cultured Tc2 cells |
| title_sort | rna seq dataset generated from stimulation of cultured tc2 cells |
| topic | Asthma Allergy Immunology |
| work_keys_str_mv | AT hoyler rnaseqdatasetgeneratedfromstimulationofculturedtc2cells |